Template:SBB10 ConstructionFiles JoseG sbb37: Difference between revisions
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(New page: Biobricking of Pcon PCR JG005-F and JG006-R on pBca1100-Bca1152. (66 bp, EcoRI/BamHI) Sub into pBjk2741-Bca1144 (EcoRI/BamHI, 910+2170, L) Product is Pcon-pBjk27...) |
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Revision as of 23:17, 8 February 2010
Biobricking of Pcon
PCR JG005-F and JG006-R on pBca1100-Bca1152. (66 bp, EcoRI/BamHI)
Sub into pBjk2741-Bca1144 (EcoRI/BamHI, 910+2170, L)
Product is Pcon-pBjk2741 {Pcon-J23100}
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JG005-F Cloning of papC ccataGAATTCgtccAGATCTttgacggctagctcagtcct
JG006-R Cloning of papC ctgatGGATCgctagcactgtacctaggac
