Template:SBB10 ConstructionFiles JoannaC sbb18
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Construction of Zinc Finger Domain (zf-) (sbb18) Pool jc01 through jc08 and jc17 through jc24, assemble by PCA PCR jc01/jc08 on PCA reaction (424 bp, EcoRI/BamHI) Sub into pBjk2741-Bca1144 (EcoRI/BamHI, 910+2170, L) Product is pBjk2741-sbb18 {<zf->} ------------ jc01 PCA Assembly of sbb17 and sbb18 AGTTCGAATTCATGAGATCTGCACCTAAGAAGAAACGCAAAGTGGGTATCC jc02 PCA Assembly of sbb17 and sbb18 GCCATTGCTGCTGGTACTCCATGGATACCCACTTTGCGTTTCTTCTT jc03 PCA Assembly of sbb17 and sbb18 TGGAGTACCAGCAGCAATGGCCGAACGTCCATTCCAGTGTCGTATTTGCA jc04 PCA Assembly of sbb17 and sbb18 TATCAGAGCGGGAGAAATTTCGCATGCAAATACGACACTGGAATGGAC jc05 PCA Assembly of sbb17 and sbb18 GCGAAAAGCCGTTCGCTTGCGACATTTGTGGACGTAAGTTTGCC jc06 PCA Assembly of sbb17 and sbb18 TCCGCATGCAGATCCGGCATTGGAAAGGTTTTTGACTACCAGTGT jc07 PCA Assembly of sbb17 and sbb18 TTCGCACTCACACAGGAGAAAAGCCTTTCGCGTGTGATATCTGCGGAC jc08 PCA Assembly of sbb17 and sbb18 GTTCAGGATCCACGGAGGTGAATTTTTGTGT jc17 PCA Assembly of sbb18 CGAAATTTCTCCCGCTCTGATAACCTGTCTGTTCACATCCGAACGCAC jc18 PCA Assembly of sbb18 GCAAGCGAACGGCTTTTCGCCAGTGTGCGTTCGGATGTGAACAGAC jc19 PCA Assembly of sbb18 ATCTTCGTATGGTTGATCCGGTGAGCGTTACGGGCAAACTTACGTCCACAAATG jc20 PCA Assembly of sbb18 TCACCGGATCAACCATACGAAGATCCACACTGGTAGTCAAAAACCTTTCCA jc21 PCA Assembly of sbb18 TGCCGGATCTGCATGCGGAATTTTAGCCGTAGTGACACCCTCAGC jc22 PCA Assembly of sbb18 GCTTTTCTCCTGTGTGAGTGCGAATATGTTCGCTGAGGGTGTCACTACGG jc23 PCA Assembly of sbb18 CGGGTTGATCGAGCTGCGAACTTTCGTCCGCAGATATCACACGCGA jc24 PCA Assembly of sbb18 TCGCAGCTCGATCAACCCGTACCAACCACACAAAAATTCACCTCCGTGGATC
JCA Notes
- Correct