Template:SBB10 ConstructionFiles JoannaC sbb18: Difference between revisions

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jc01    PCA Assembly of sbb17 and sbb18    AGTTCGAATTCATCAGATCTGCTCCGAAAAAAAAACGTAAAGTTGGTATCC
jc01    PCA Assembly of sbb17 and sbb18    AGTTCGAATTCATGAGATCTGCACCTAAGAAGAAACGCAAAGTGGGTATCC
jc02    PCA Assembly of sbb17 and sbb18    CATAGCAGCCGGAACACCGTGGATACCAACTTTACGTTTTTTTTT
jc02    PCA Assembly of sbb17 and sbb18    GCCATTGCTGCTGGTACTCCATGGATACCCACTTTGCGTTTCTTCTT
jc03    PCA Assembly of sbb17 and sbb18    CGGTGTTCCGGCTGCTATGGCTGAACGTCCGTTCCAGTGCCGTATC
jc03    PCA Assembly of sbb17 and sbb18    TGGAGTACCAGCAGCAATGGCCGAACGTCCATTCCAGTGTCGTATTTGCA
jc04    PCA Assembly of sbb17 and sbb18    TGTCAGAACGAGAGAAGTTACGCATGCAGATACGGCACTGGAACGGAC
jc04    PCA Assembly of sbb17 and sbb18    TATCAGAGCGGGAGAAATTTCGCATGCAAATACGACACTGGAATGGAC
jc05    PCA Assembly of sbb17 and sbb18    GTGAAAAACCGTTCGCTTGCGACATCTGCGGTCGTAAATTtGCa
jc05    PCA Assembly of sbb17 and sbb18    GCGAAAAGCCGTTCGCTTGCGACATTTGTGGACGTAAGTTTGCC
jc06    PCA Assembly of sbb17 and sbb18    GAAGTTACGCATGCAGATACGGCACTGGAACGGTTTCTGAGAACCTGTGT
jc06    PCA Assembly of sbb17 and sbb18    TCCGCATGCAGATCCGGCATTGGAAAGGTTTTTGACTACCAGTGT
jc07    PCA Assembly of sbb17 and sbb18    TCCGTACCCACACAGGTGAAAAACCGTTtGCaTGCGAtATCTGCGG
jc07    PCA Assembly of sbb17 and sbb18    TTCGCACTCACACAGGAGAAAAGCCTTTCGCGTGTGATATCTGCGGAC
jc08    PCA Assembly of sbb17 and sbb18    GTTCAGGATCCACGCAGGTGGATTTTGGTGT
jc08    PCA Assembly of sbb17 and sbb18    GTTCAGGATCCACGGAGGTGAATTTTTGTGT
jc17    PCA Assembly of sbb18              CGTAACTTCTCTCGTTCTGACAACCTGTCTGTTCACATCCGTACCCAC
jc17    PCA Assembly of sbb18              CGAAATTTCTCCCGCTCTGATAACCTGTCTGTTCACATCCGAACGCAC
jc18    PCA Assembly of sbb18              GTCGCAAGCGAACGGTTTTTCACCTGTGTGGGTACGGATGTGAACAGAC
jc18    PCA Assembly of sbb18              GCAAGCGAACGGCTTTTCGCCAGTGTGCGTTCGGATGTGAACAGAC
jc19    PCA Assembly of sbb18              GATTTTGGTGTGGTTGATACGGTGAGCGTTACGtGCaAATTTACGACCGCAG
jc19    PCA Assembly of sbb18              ATCTTCGTATGGTTGATCCGGTGAGCGTTACGGGCAAACTTACGTCCACAAATG
jc20    PCA Assembly of sbb18              TCACCGTATCAACCACACCAAAATCCACACAGGTTCTCAGAAACCGTTCC
jc20    PCA Assembly of sbb18              TCACCGGATCAACCATACGAAGATCCACACTGGTAGTCAAAAACCTTTCCA
jc21    PCA Assembly of sbb18              TGCCGTATCTGCATGCGTAACTTCTCTCGTTCTGACACCCTGTCTGAAC
jc21    PCA Assembly of sbb18              TGCCGGATCTGCATGCGGAATTTTAGCCGTAGTGACACCCTCAGC
jc22    PCA Assembly of sbb18              GTTTTTCACCTGTGTGGGTACGGATGTGTTCAGACAGGGTGTCAGAACGA
jc22    PCA Assembly of sbb18              GCTTTTCTCCTGTGTGAGTGCGAATATGTTCGCTGAGGGTGTCACTACGG
jc23    PCA Assembly of sbb18              CGGGTAGAACGAGCAGCGAAcTTACGtCCGCAGATaTCGCAtGCaA
jc23    PCA Assembly of sbb18              CGGGTTGATCGAGCTGCGAACTTTCGTCCGCAGATATCACACGCGA
jc24    PCA Assembly of sbb18              TCGCTGCTCGTTCTACCCGTACCAACCACACCAAAATCCACCTGCGTGG
jc24    PCA Assembly of sbb18              TCGCAGCTCGATCAACCCGTACCAACCACACAAAAATTCACCTCCGTGGATC
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Revision as of 03:03, 11 February 2010

Construction of Zinc Finger Domain (zf-) (sbb18)

Pool jc01 through jc08 and jc17 through jc24, assemble by PCA
PCR jc01/jc08 on PCA reaction	(424 bp, EcoRI/BamHI)
Sub into pBjk2741-Bca1144	(EcoRI/BamHI, 910+2170, L)
Product is pBjk2741-sbb18       {<zf->}

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jc01     PCA Assembly of sbb17 and sbb18     AGTTCGAATTCATGAGATCTGCACCTAAGAAGAAACGCAAAGTGGGTATCC
jc02     PCA Assembly of sbb17 and sbb18     GCCATTGCTGCTGGTACTCCATGGATACCCACTTTGCGTTTCTTCTT
jc03     PCA Assembly of sbb17 and sbb18     TGGAGTACCAGCAGCAATGGCCGAACGTCCATTCCAGTGTCGTATTTGCA
jc04     PCA Assembly of sbb17 and sbb18     TATCAGAGCGGGAGAAATTTCGCATGCAAATACGACACTGGAATGGAC
jc05     PCA Assembly of sbb17 and sbb18     GCGAAAAGCCGTTCGCTTGCGACATTTGTGGACGTAAGTTTGCC
jc06     PCA Assembly of sbb17 and sbb18     TCCGCATGCAGATCCGGCATTGGAAAGGTTTTTGACTACCAGTGT
jc07     PCA Assembly of sbb17 and sbb18     TTCGCACTCACACAGGAGAAAAGCCTTTCGCGTGTGATATCTGCGGAC
jc08     PCA Assembly of sbb17 and sbb18     GTTCAGGATCCACGGAGGTGAATTTTTGTGT
jc17     PCA Assembly of sbb18               CGAAATTTCTCCCGCTCTGATAACCTGTCTGTTCACATCCGAACGCAC
jc18     PCA Assembly of sbb18               GCAAGCGAACGGCTTTTCGCCAGTGTGCGTTCGGATGTGAACAGAC
jc19     PCA Assembly of sbb18               ATCTTCGTATGGTTGATCCGGTGAGCGTTACGGGCAAACTTACGTCCACAAATG
jc20     PCA Assembly of sbb18               TCACCGGATCAACCATACGAAGATCCACACTGGTAGTCAAAAACCTTTCCA
jc21     PCA Assembly of sbb18               TGCCGGATCTGCATGCGGAATTTTAGCCGTAGTGACACCCTCAGC
jc22     PCA Assembly of sbb18               GCTTTTCTCCTGTGTGAGTGCGAATATGTTCGCTGAGGGTGTCACTACGG
jc23     PCA Assembly of sbb18               CGGGTTGATCGAGCTGCGAACTTTCGTCCGCAGATATCACACGCGA
jc24     PCA Assembly of sbb18               TCGCAGCTCGATCAACCCGTACCAACCACACAAAAATTCACCTCCGTGGATC
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