Template:SBB10 ConstructionFiles JoannaC sbb18: Difference between revisions
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Joanna Chen (talk | contribs) (New page: <pre> Construction of Zinc Finger Domain (zf-) (sbb18) Pool jc01 through jc08 and jc17 through jc24, assemble by PCA PCR jc01/jc08 on PCA reaction (424 bp, EcoRI/BamHI) Sub into pBjk2741-...) |
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Revision as of 02:57, 9 February 2010
Construction of Zinc Finger Domain (zf-) (sbb18)
Pool jc01 through jc08 and jc17 through jc24, assemble by PCA
PCR jc01/jc08 on PCA reaction (424 bp, EcoRI/BamHI)
Sub into pBjk2741-Bca1144 (EcoRI/BamHI, 910+2170, L)
Product is pBjk2741-sbb18 {<zf->}
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jc01 PCA Assembly of sbb17 and sbb18 AGTTCGAATTCATCAGATCTGCTCCGAAAAAAAAACGTAAAGTTGGTATCC
jc02 PCA Assembly of sbb17 and sbb18 CATAGCAGCCGGAACACCGTGGATACCAACTTTACGTTTTTTTTT
jc03 PCA Assembly of sbb17 and sbb18 CGGTGTTCCGGCTGCTATGGCTGAACGTCCGTTCCAGTGCCGTATC
jc04 PCA Assembly of sbb17 and sbb18 TGTCAGAACGAGAGAAGTTACGCATGCAGATACGGCACTGGAACGGAC
jc05 PCA Assembly of sbb17 and sbb18 GTGAAAAACCGTTCGCTTGCGACATCTGCGGTCGTAAATTCGCT
jc06 PCA Assembly of sbb17 and sbb18 GAAGTTACGCATGCAGATACGGCACTGGAACGGTTTCTGAGAACCTGTGT
jc07 PCA Assembly of sbb17 and sbb18 TCCGTACCCACACAGGTGAAAAACCGTTCGCTTGCGACATCTGCGG
jc08 PCA Assembly of sbb17 and sbb18 GTTCAGGATCCACGCAGGTGGATTTTGGTGT
jc17 PCA Assembly of sbb18 CGTAACTTCTCTCGTTCTGACAACCTGTCTGTTCACATCCGTACCCAC
jc18 PCA Assembly of sbb18 GTCGCAAGCGAACGGTTTTTCACCTGTGTGGGTACGGATGTGAACAGAC
jc19 PCA Assembly of sbb18 GATTTTGGTGTGGTTGATACGGTGAGCGTTACGAGCGAATTTACGACCGCAG
jc20 PCA Assembly of sbb18 TCACCGTATCAACCACACCAAAATCCACACAGGTTCTCAGAAACCGTTCC
jc21 PCA Assembly of sbb18 TGCCGTATCTGCATGCGTAACTTCTCTCGTTCTGACACCCTGTCTGAAC
jc22 PCA Assembly of sbb18 GTTTTTCACCTGTGTGGGTACGGATGTGTTCAGACAGGGTGTCAGAACGA
jc23 PCA Assembly of sbb18 CGGGTAGAACGAGCAGCGAATTTACGACCGCAGATGTCGCAAGCGA
jc24 PCA Assembly of sbb18 TCGCTGCTCGTTCTACCCGTACCAACCACACCAAAATCCACCTGCGTGG
