Template:SBB10 ConstructionFiles JoannaC sbb17: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Joanna Chen (talk | contribs) (New page: <pre> Construction of Zinc Finger Domain (zf+) (sbb17) Pool jc01 through jc16, assemble by PCA PCR jc01/jc08 on PCA reaction (424 bp, EcoRI/BamHI) Sub into pBjk2741-Bca1144 (EcoRI/BamHI, ...) |
(No difference)
|
Revision as of 02:54, 9 February 2010
Construction of Zinc Finger Domain (zf+) (sbb17)
Pool jc01 through jc16, assemble by PCA
PCR jc01/jc08 on PCA reaction (424 bp, EcoRI/BamHI)
Sub into pBjk2741-Bca1144 (EcoRI/BamHI, 910+2170, L)
Product is pBjk2741-sbb17 {<zf+>}
------------
jc01 PCA Assembly of sbb17 and sbb18 AGTTCGAATTCATCAGATCTGCTCCGAAAAAAAAACGTAAAGTTGGTATCC
jc02 PCA Assembly of sbb17 and sbb18 CATAGCAGCCGGAACACCGTGGATACCAACTTTACGTTTTTTTTT
jc03 PCA Assembly of sbb17 and sbb18 CGGTGTTCCGGCTGCTATGGCTGAACGTCCGTTCCAGTGCCGTATC
jc04 PCA Assembly of sbb17 and sbb18 TGTCAGAACGAGAGAAGTTACGCATGCAGATACGGCACTGGAACGGAC
jc05 PCA Assembly of sbb17 and sbb18 GTGAAAAACCGTTCGCTTGCGACATCTGCGGTCGTAAATTCGCT
jc06 PCA Assembly of sbb17 and sbb18 GAAGTTACGCATGCAGATACGGCACTGGAACGGTTTCTGAGAACCTGTGT
jc07 PCA Assembly of sbb17 and sbb18 TCCGTACCCACACAGGTGAAAAACCGTTCGCTTGCGACATCTGCGG
jc08 PCA Assembly of sbb17 and sbb18 GTTCAGGATCCACGCAGGTGGATTTTGGTGT
jc09 PCA Assembly of sbb17 CGTAACTTCTCTCGTTCTGACACCCTGTCTGAACACATCCGTACCCAC
jc10 PCA Assembly of sbb17 GTCGCAAGCGAACGGTTTTTCACCTGTGTGGGTACGGATGTGTTCAGAC
jc11 PCA Assembly of sbb17 GGTGTGGGTGGTACGGGTAGAACGAGCAGCGAATTTACGACCGCAG
jc12 PCA Assembly of sbb17 TACCCGTACCACCCACACCAAAATCCACACAGGTTCTCAGAAACCGTTCC
jc13 PCA Assembly of sbb17 TGCCGTATCTGCATGCGTAACTTCTCTCGTTCTGACTCTCTGTCTAAACAC
jc14 PCA Assembly of sbb17 GTTTTTCACCTGTGTGGGTACGGATGTGTTTAGACAGAGAGTCAGAACGA
jc15 PCA Assembly of sbb17 CTTTCAGGTTAGAACGCTGAGCGAATTTACGACCGCAGATGTCGCAAGCGA
jc16 PCA Assembly of sbb17 TCGCTCAGCGTTCTAACCTGAAAGTTCACACCAAAATCCACCTGCGTGG
