Template:SBB10 ConstructionFiles JennaK sbb42

Biobricking of pre-pro sequence basic part sbb42

Digest pBjh1601AC-Bjh1723                      (BglII/BamHI, 91+3203, S)
Sub into pBca9523-Bca1144                      (EcoRI/BamHI, 918+2472, L)
Product is pBca9523-rbs_pelB  	{rbs_pelB>}

JCA Notes

• You're going to be digesting EcoRI/BamHI for both source and destination
• You want to transfer into pBjk2471
• Product has same partName as source

Eco/Bam transfer pBjh1601AC-Bjh1723
Sub into pBjk2741-Bca1144            (EcoRI/BamHI, 910+2170, L)
Product is pBjk2741-Bjh1723  	{rbs_pelB>}