Template:SBB10 ConstructionFiles AndrewS sbb12
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1) sbb12: Sleeping Beauty 3'TR ---------------------------------------------------------- Pool AS001-F through AS008-R, assemble by PCA PCR AS001-F/AS008-R on PCA reaction (259 bp, EcoRI/BamHI) Substitute in pBjk2741-Bca1144 (EcoRI/BamHI, 910+2170, L) Product is pBjk2741-sbb12 ---------------------------------------------------------- AS001-F PCA Assembly of sbb12 CCATAGAATTCATGAGATCTTTGAGTGTATGTTAACTTCTGACCCACTGG AS002-R PCA Assembly of sbb12 ATTTCAGCTTTTATTTCTTTCATCACATTCCCAGTGGGTCAGAAGTTAAC AS003-F PCA Assembly of sbb12 AAAGAAATAAAAGCTGAAATGAATCATTCTCTCTACTATTATTCTGATAT AS004-R PCA Assembly of sbb12 AGGATCACCACTTTATTTTAAGAATGTGAAATATCAGAATAATAGTAGAG AS005-F PCA Assembly of sbb12 TAAAATAAAGTGGTGATCCTAACTGACCTTAAGACAGGGAATCTTTACTC AS006-R PCA Assembly of sbb12 CACTTTTTCACAATTCCTGACATTTAATCCGAGTAAAGATTCCCTGTCTT AS007-F PCA Assembly of sbb12 TCAGGAATTGTGAAAAAGTGAGTTTAATGTATTTGGCTAAGGTGTATGTA AS008-R PCA Assembly of sbb12 CTGATGGATCCCAGTTGAAGTCGGAAGTTTACATACACCTTAGCCAAAT
JCA Notes
- correct
- Need to put parts in clotho
