Template:SBB-Protocols Assay4: Difference between revisions
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http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=111355 PMCID: PMC111355 <br> | http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=111355 PMCID: PMC111355 <br> | ||
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=193771 PMCID: PMC193771 <br> | http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=193771 PMCID: PMC193771 <br> | ||
1. Seed cultures in V-bottom plate | 1. Seed cultures in V-bottom plate <br> | ||
2. Identify colonies that display prominent flocculation | 2. Identify colonies that display prominent flocculation <br> | ||
3. Proceed with quantitative measurement for chosen colonies | 3. Proceed with quantitative measurement for chosen colonies <br> | ||
4. Negative Control: pBca9495CA-Bca1363, Positive Control: pBca9145-Bca9494 | 4. Negative Control: pBca9495CA-Bca1363, Positive Control: pBca9145-Bca9494 <br> | ||
5. Grow select colonies and controls to an OD of 0.5 at | 5. Grow select colonies and controls to an OD of 0.5 at 600nm <br> | ||
6. Add X .5 mM IPTG and shake for 3 hours at 37°C | 6. Add X .5 mM IPTG and shake for 3 hours at 37°C <br> | ||
7. Transfer 200 uL to 96-well plate and take samples at 20 minute intervals | 7. Transfer 200 uL to 96-well plate and take samples at 20 minute intervals <br> | ||
Revision as of 11:58, 15 April 2009
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=111355 PMCID: PMC111355
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=193771 PMCID: PMC193771
1. Seed cultures in V-bottom plate
2. Identify colonies that display prominent flocculation
3. Proceed with quantitative measurement for chosen colonies
4. Negative Control: pBca9495CA-Bca1363, Positive Control: pBca9145-Bca9494
5. Grow select colonies and controls to an OD of 0.5 at 600nm
6. Add X .5 mM IPTG and shake for 3 hours at 37°C
7. Transfer 200 uL to 96-well plate and take samples at 20 minute intervals
