Template:SBB-Protocols Assay1: Difference between revisions
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(New page: =='''Growth Assay Protocol'''== 1. Replate 16 constructs on new plates at the same time. 2. Plate DH10B, pBca9145-Bca9494, and pBca9495CA-Bca1144 at the same time. 3. Grow plates overnigh...) |
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=='''Growth Assay Protocol'''== | =='''Growth Assay Protocol'''== | ||
* Replate 16 constructs on new plates at the same time. | |||
* Plate DH10B, pBca9145-Bca9494, and pBca9495CA-Bca1144 at the same time. | |||
* Grow plates overnight. | |||
* Pick 10 colonies from each. | |||
* Grow to saturation in 96 well blocks with 400 uL LB media in each well. | |||
* Add 50 uL of LB media per well in 384 well plate. | |||
* Add 1 uL of cell sample to each well. | |||
* Add 1000x Arabinose to 5 out of the 10 wells for each construct. | |||
* Place plate in Tecan and run OD measurements every 10 minutes. | |||
Revision as of 11:32, 15 April 2009
Growth Assay Protocol
- Replate 16 constructs on new plates at the same time.
- Plate DH10B, pBca9145-Bca9494, and pBca9495CA-Bca1144 at the same time.
- Grow plates overnight.
- Pick 10 colonies from each.
- Grow to saturation in 96 well blocks with 400 uL LB media in each well.
- Add 50 uL of LB media per well in 384 well plate.
- Add 1 uL of cell sample to each well.
- Add 1000x Arabinose to 5 out of the 10 wells for each construct.
- Place plate in Tecan and run OD measurements every 10 minutes.
