Template:PhiC31 Assay Protocol

1. transform cells with plasmid containing phiC31 cds+attB/attP site Transformation Protocol
2. inoculate and grow up a colony in 3ml media
3. incubate with arabinose to a concentration of 1% arabinose for 15 minutes
4. miniprep cells Miniprep Protocol
5. transform purified plasmid into cells
6. plate on selective media
7. observe GFP expression
8. select a white colony and inoculate it
9. miniprep the grown up colony
10. sequence the plasmid