Template:ASUBME103 s2014 L6: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> BME 100 Spring 2014</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> BME 100 Spring 2015</span>
|style="background-color: #F2F2F2" | [[BME100_s2014 | <font face="trebuchet ms" style="color: #808080"> '''Home''' </font>]]<br>[[BME100_s2014:People | <font face="trebuchet ms" style="color: #808080"> '''People''' </font>]]<br>[[BME100_s2014:Projects1 | <font face="trebuchet ms" style="color: #808080"> '''Lab Write-Up 1''' </font>]] | [[BME100_s2014:Projects2 | <font face="trebuchet ms" style="color: #808080"> '''Lab Write-Up 2''' </font>]] | [[BME100_s2014:Projects3 | <font face="trebuchet ms" style="color: #808080"> '''Lab Write-Up 3''' </font>]]<br>[[BME100_s2014:Projects4 | <font face="trebuchet ms" style="color: #808080"> '''Lab Write-Up 4''' </font>]] | [[BME100_s2014:Projects5 | <font face="trebuchet ms" style="color: #808080"> '''Lab Write-Up 5''' </font>]] | [[BME100_s2014:Projects6 | <font face="trebuchet ms" style="color: #808080"> '''Lab Write-Up 6''' </font>]]<br>[[BME100_s2014:Logistics | <font face="trebuchet ms" style="color: #808080"> ''' Course Logistics For Instructors''' </font>]] <br>[[BME100_s2014:Photos | <font face="trebuchet ms" style="color: #808080"> '''Photos''' </font>]] <br>[[BME100_s2014:WikiHelp | <font face="trebuchet ms" style="color: #808080"> '''Wiki Editing Help''' </font>]]  
|style="background-color: #F2F2F2" | [[BME100_s2015 | <font face="trebuchet ms" style="color: #808080"> '''Home''' </font>]]<br>[[BME100_s2015:People | <font face="trebuchet ms" style="color: #808080"> '''People''' </font>]]<br>[[BME100_s2015:Projects1 | <font face="trebuchet ms" style="color: #808080"> '''Lab Write-Up 1''' </font>]] | [[BME100_s2015:Projects2 | <font face="trebuchet ms" style="color: #808080"> '''Lab Write-Up 2''' </font>]] | [[BME100_s2015:Projects3 | <font face="trebuchet ms" style="color: #808080"> '''Lab Write-Up 3''' </font>]]<br>[[BME100_s2015:Projects4 | <font face="trebuchet ms" style="color: #808080"> '''Lab Write-Up 4''' </font>]] | [[BME100_s2015:Projects5 | <font face="trebuchet ms" style="color: #808080"> '''Lab Write-Up 5''' </font>]] | [[BME100_s2015:Projects6 | <font face="trebuchet ms" style="color: #808080"> '''Lab Write-Up 6''' </font>]]<br>[[BME100_s2015:Logistics | <font face="trebuchet ms" style="color: #808080"> ''' Course Logistics For Instructors''' </font>]] <br>[[BME100_s2015:Photos | <font face="trebuchet ms" style="color: #808080"> '''Photos''' </font>]] <br>[[BME100_s2015:WikiHelp | <font face="trebuchet ms" style="color: #808080"> '''Wiki Editing Help''' </font>]]  
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| style="background-color: #ffcc66; padding: 5px;" colspan="2" | [[Image:BME494_Asu_logo.png]]
| style="background-color: #ffcc66; padding: 5px;" colspan="2" | [[Image:BME494_Asu_logo.png]]
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'''TinkerCAD'''<br>
'''TinkerCAD'''<br>


''[Instructions: A short summary (up to five sentences) of the TinkerCAD tool and how you used it in lab on November 20th]''<br>
''[Instructions: A short summary (up to five sentences) of the TinkerCAD tool and how you used it during the Computer-Aided Design lab]''<br>


''[Instructions: Show an image of your TinkerCAD PCR tube design here]''


'''Our Design'''<br>


'''Implications of Using TinkerCAD for Design'''<br>
''[Instructions: Show an image of your TinkerCAD design here]''


''[Instructions: A short paragraph discussing just one possible way to use TinkerCAD for something practical...like redesigning the OpenPCR machine, fluorimeter, camera holder, printing out some of the smaller plastic items on demand, etc. There are lots of possibilities...pick just ONE.]''<br>
''[Instructions: A short paragraph describing your design. Why did you choose this design? How is it different from the original OpenPCR design?]''<br>




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<!-- Note: Be sure to delete the text in brackets: ''[ ]'' -->
<!-- Note: Be sure to delete the text in brackets: ''[ ]'' -->


==Feature 1: Cancer SNP-Specific Primers==
==Feature 1: Disease SNP-Specific Primers==


''[Instructions: This information will come from the Week 9 exercises you did in lab. Your notes should be in a pdf file that is saved on Blackboard under your group.]''
''[Instructions: This information will come from the exercises you did in PCR Lab B.]''


'''Background on the cancer-associated mutation'''<br>
'''Background on the disease-associated mutation'''<br>


''[Instructions: Use the answers from questions 3, 4, 5, and 7 to compose, in your own words, a paragraph about rs17879961]''
''[Instructions: Use the answers from questions 3 - 7 to compose, in your own words, a paragraph about rs237025]''




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'''Primer design'''<br>
'''Primer design'''<br>


* Forward Primer: ''[Instructions: write the sequence of the forward primer]''
* Disease SNP-specific Forward Primer: ''[Instructions: type the sequence of the forward primer]''
* Cancer-specific Reverse Primer: ''[Instructions: write the sequence of the forward primer]''
* Reverse Primer: ''[Instructions: type the sequence of the reverse primer]''


How the primers work: ''[Instructions: explain what makes the primers cancer-sequence specific. In other words, explain why the primers will amplify DNA that contains the cancer-associated SNP rs17879961, and will not exponentially amplify DNA that has the non-cancer allele.]''
How the primers work: ''[Instructions: explain what makes the primers disease-sequence specific. In other words, explain why the primers will amplify DNA that contains the disease-associated SNP, and will not exponentially amplify DNA that has the non-disease allele.]''




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''[Instructions: Summarize how the consumables will be packaged in your kit. You may add a schematic image. An image is OPTIONAL and will not get bonus points, but it will make your report look awesome and easy to score.]''
''[Instructions: Summarize how the consumables will be packaged in your kit. You may add a schematic image. An image is OPTIONAL and will not get bonus points, but it will make your report look awesome and easy to score.]''


''[Instructions: IF your consumables packaging plan addresses any major weakness discussed by your group or mentioned by others (see the Virtual Comment Board Powerpoint files on Blackboard, Lab Week 12) explain how in an additional paragraph.]''
''[Instructions: IF your consumables packaging plan addresses any major weakness(es), explain how in an additional paragraph.]''




<!-- Note: Be sure to delete the text in brackets: ''[ ]'' -->
<!-- Note: Be sure to delete the text in brackets: ''[ ]'' -->


==Feature 3: Hardware - PCR Machine & Fluorimeter==


==Feature 3: PCR Machine Hardware==
''[Instructions: Summarize how you will include the PCR machine and fluorimeter in your system. You may add a schematic image. An image is OPTIONAL and will not get bonus points, but it will make your report look really awesome and easy to score.]''


''[Instructions: Summarize how you will include the PCR machine in your system. You may add a schematic image. An image is OPTIONAL and will not get bonus points, but it will make your report look really awesome and easy to score.]''
''[Instructions: IF your group has decided to redesign the PCR machine and/or Fluorimeter to address any major weakness(es), explain how in an additional paragraph.]''
 
''[Instructions: IF your group has decided to redesign the PCR machine to address any major weakness discussed by your group or mentioned by others (see the Virtual Comment Board Powerpoint files on Blackboard, Lab Week 12) explain how in an additional paragraph.]''
 
 
<!-- Note: Be sure to delete the text in brackets: ''[ ]'' -->
 
==Feature 4: Fluorimeter Hardware==
 
''[Instructions: Summarize how you will include the fluorimeter in your system. You may add a schematic image. An image is OPTIONAL and will not get bonus points, but it will make your report look really REALLY awesome and easy to score.]''
 
''[Instructions: IF your group has decided to redesign the fluorimeter to address any major weakness discussed by your group or mentioned by others (see the Virtual Comment Board Powerpoint files on Blackboard, Lab Week 12) explain how in an additional paragraph.]''




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==Bonus Opportunity: What Bayesian Stats Imply About The BME100 Diagnostic Approach==
==Bonus Opportunity: What Bayesian Stats Imply About The BME100 Diagnostic Approach==


''[Instructions: This section is OPTIONAL, and will get bonus points if answered thoroughly and correctly. Here is a chance to flex some intellectual muscle. In your own words, discuss what the results for calculations 3 and 4 imply about the reliability of CHEK2 PCR for predicting cancer. Please do NOT type the actual numerical values here. Just refer to them as being "less than one" or "very small." The instructors will ask you to submit your actual calculations via a Blackboard quiz. We are doing so for the sake of academic integrity and to curb any temptation to cheat.]''
''[Instructions: This section is OPTIONAL, and will get bonus points if answered thoroughly and correctly. Here is a chance to flex some intellectual muscle. In your own words, discuss what the results for calculations 3 and 4 imply about the reliability of PCR for predicting the disease. Please do NOT type the actual numerical values here. Just refer to them as being "close to one" or "very small." The instructors will ask you to submit your actual calculations via a Blackboard quiz. We are doing so for the sake of academic integrity and to curb any temptation to cheat.]''





Latest revision as of 11:11, 5 January 2015

BME 100 Spring 2015 Home
People
Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
Course Logistics For Instructors
Photos
Wiki Editing Help


OUR COMPANY

Name: student
Name: student
Name: student
Name: student
Name: student
Name: student


[Instructions: add the name of your team's company and/or product here]


LAB 6 WRITE-UP

Computer-Aided Design

TinkerCAD

[Instructions: A short summary (up to five sentences) of the TinkerCAD tool and how you used it during the Computer-Aided Design lab]


Our Design

[Instructions: Show an image of your TinkerCAD design here]

[Instructions: A short paragraph describing your design. Why did you choose this design? How is it different from the original OpenPCR design?]



Feature 1: Disease SNP-Specific Primers

[Instructions: This information will come from the exercises you did in PCR Lab B.]

Background on the disease-associated mutation

[Instructions: Use the answers from questions 3 - 7 to compose, in your own words, a paragraph about rs237025]


Primer design

  • Disease SNP-specific Forward Primer: [Instructions: type the sequence of the forward primer]
  • Reverse Primer: [Instructions: type the sequence of the reverse primer]

How the primers work: [Instructions: explain what makes the primers disease-sequence specific. In other words, explain why the primers will amplify DNA that contains the disease-associated SNP, and will not exponentially amplify DNA that has the non-disease allele.]



Feature 2: Consumables Kit

[Instructions: Summarize how the consumables will be packaged in your kit. You may add a schematic image. An image is OPTIONAL and will not get bonus points, but it will make your report look awesome and easy to score.]

[Instructions: IF your consumables packaging plan addresses any major weakness(es), explain how in an additional paragraph.]


Feature 3: Hardware - PCR Machine & Fluorimeter

[Instructions: Summarize how you will include the PCR machine and fluorimeter in your system. You may add a schematic image. An image is OPTIONAL and will not get bonus points, but it will make your report look really awesome and easy to score.]

[Instructions: IF your group has decided to redesign the PCR machine and/or Fluorimeter to address any major weakness(es), explain how in an additional paragraph.]


Bonus Opportunity: What Bayesian Stats Imply About The BME100 Diagnostic Approach

[Instructions: This section is OPTIONAL, and will get bonus points if answered thoroughly and correctly. Here is a chance to flex some intellectual muscle. In your own words, discuss what the results for calculations 3 and 4 imply about the reliability of PCR for predicting the disease. Please do NOT type the actual numerical values here. Just refer to them as being "close to one" or "very small." The instructors will ask you to submit your actual calculations via a Blackboard quiz. We are doing so for the sake of academic integrity and to curb any temptation to cheat.]