Template:ASUBME103 s2014 L5: Difference between revisions
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'''Representative Images of Samples''' | '''Representative Images of Samples''' | ||
<!-- INSTRUCTIONS: Show an IMAGE <u>where you drew a circle around the droplet</u> with the freehand tool for a sample with no DNA. --> | |||
''[Instructions: Show an IMAGE <u>where you drew a circle around the droplet</u> with the freehand tool for a sample '''with''' DNA (positive signal)]'' | ''[Instructions: Show an IMAGE <u>where you drew a circle around the droplet</u> with the freehand tool for a sample '''with''' DNA (positive signal)]'' | ||
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'''Image J Values for All Samples''' | '''Image J Values for All Samples''' | ||
<!-- INSTRUCTIONS: See worksheet page 8. '''To save time on typing a new Wiki table from scratch''', use THIS TOOL to auto-generate a Wiki table: [http://excel2wiki.net/wikipedia.php Excel-to-Wiki Converter]. Copy the headers and values from the Excel spreadsheet you made, paste them into the form field, click submit, copy the Wiki code that the tool generated, and replace TABLE GOES HERE (below) with your auto-generated code. --> | |||
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'''Fitting a Straight Line'''<br> | '''Fitting a Straight Line'''<br> | ||
<!-- INSTRUCTIONS: Place an image of your Excel plot with a line of best fit here. --> | |||
'''PCR Results Summary''' | '''PCR Results Summary''' | ||
<!-- INSTRUCTIONS: You completed 8 PCR reactions | <!-- INSTRUCTIONS: You completed 8 PCR reactions and used the SYBR Green I staining and imaging technique to measure the amount of amplified DNA in each PCR reaction. You used a standard curve (based on known concentrations of calf thymus DNA) to convert INTDEN values into DNA concentration. Your positive control and negative control samples should be used as '''threshold''' values for determining whether an unknown (patient) sample is truly positive or negative. Replace the underscore with your claculated initial concentration values.--> | ||
* Our positive control PCR result was ____ μg/mL | * Our positive control PCR result was ____ μg/mL | ||
* Our negative control PCR result was ____ μg/mL | * Our negative control PCR result was ____ μg/mL | ||
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* Patient _____ : | * Patient _____ : | ||
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Revision as of 08:09, 29 October 2014
BME 100 Spring 2014 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||||||
OUR TEAM
LAB 5 WRITE-UPBackground InformationSYBR Green Dye
ProcedureSmart Phone Camera Settings
[Instructions: If you used an additional phone, describe the other type of smart phone you used and how you adjusted the camera settings, if applicable. If not, delete this part]
[Instructions: Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.]
[Instructions: See worksheet page 6.]
[Add more rows as needed]
Data AnalysisRepresentative Images of Samples [Instructions: Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)]
Image J Values for All Samples
Observed results
Conclusions
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