Template:ASUBME103 s2014 L5: Difference between revisions

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'''Representative Images of Samples'''
'''Representative Images of Samples'''
 
<!-- INSTRUCTIONS: Show an IMAGE <u>where you drew a circle around the droplet</u> with the freehand tool for a sample with no DNA. -->
''[Instructions: Show an IMAGE <u>where you drew a circle around the droplet</u> with the freehand tool for a sample with no DNA]''


''[Instructions: Show an IMAGE <u>where you drew a circle around the droplet</u> with the freehand tool for a sample '''with''' DNA (positive signal)]''
''[Instructions: Show an IMAGE <u>where you drew a circle around the droplet</u> with the freehand tool for a sample '''with''' DNA (positive signal)]''
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'''Image J Values for All Samples'''  
'''Image J Values for All Samples'''  
 
<!-- INSTRUCTIONS: See worksheet page 8. '''To save time on typing a new Wiki table from scratch''', use THIS TOOL to auto-generate a Wiki table: [http://excel2wiki.net/wikipedia.php Excel-to-Wiki Converter]. Copy the headers and values from the Excel spreadsheet you made, paste them into the form field, click submit, copy the Wiki code that the tool generated, and replace TABLE GOES HERE (below) with your auto-generated code. -->
''[Instructions: See worksheet page 8. '''To save time on typing a new Wiki table from scratch''', use THIS TOOL to auto-generate a Wiki table: [http://excel2wiki.net/wikipedia.php Excel-to-Wiki Converter]. Copy the headers and values from the Excel spreadsheet you made, paste them into the form field, click submit, copy the Wiki code that the tool generated, and replace TABLE GOES HERE (below) with your auto-generated code.]''




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'''Fitting a Straight Line'''<br>
'''Fitting a Straight Line'''<br>
<!-- INSTRUCTIONS: Place an image of your Excel plot with a line of best fit here. -->


''[Instructions: Place an IMAGE of your Excel plot with a line of best fit here. See worksheet page 9]''


'''PCR Results Summary'''
'''PCR Results Summary'''
<!-- INSTRUCTIONS: You completed 8 PCR reactions in a previous lab. You used the SYBR Green I staining and imaging technique to measure the amount of amplified DNA in each PCR reaction. You used a standard curve (based on known concentrations of calf thymus DNA) to convert INTDEN values into DNA concentration. Your positive control and negative control samples should be used as '''threshold''' values for determining whether an unknown (patient) sample is truly positive or negative. -->
<!-- INSTRUCTIONS: You completed 8 PCR reactions and used the SYBR Green I staining and imaging technique to measure the amount of amplified DNA in each PCR reaction. You used a standard curve (based on known concentrations of calf thymus DNA) to convert INTDEN values into DNA concentration. Your positive control and negative control samples should be used as '''threshold''' values for determining whether an unknown (patient) sample is truly positive or negative. Replace the underscore with your claculated initial concentration values.-->
* Our positive control PCR result was ____ μg/mL
* Our positive control PCR result was ____ μg/mL
* Our negative control PCR result was ____ μg/mL
* Our negative control PCR result was ____ μg/mL
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* Patient _____ :
* Patient _____ :


<br>
<!-- Note: Be sure to delete the text in brackets: ''[ ]'' -->




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Revision as of 08:09, 29 October 2014

BME 100 Spring 2014 Home
People
Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
Course Logistics For Instructors
Photos
Wiki Editing Help


OUR TEAM

Name: student
Name: student
Name: student
Name: student
Name: student
Name: student


LAB 5 WRITE-UP

Background Information

SYBR Green Dye
[Instructions: A short summary describing SYBR green dye]


Single-Drop Fluorimeter
[Instructions: A description of the single-drop fluorimeter device. Add a PHOTO for bonus points]


How the Fluorescence Technique Works
[Instructions: In your own words]



Procedure

Smart Phone Camera Settings
[Instructions: The type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).]

  • Type of Smartphone:
    • Flash:
    • ISO setting:
    • White Balance:
    • Exposure:
    • Saturation:
    • Contrast:

[Instructions: If you used an additional phone, describe the other type of smart phone you used and how you adjusted the camera settings, if applicable. If not, delete this part]

  • Type of Smartphone:
    • Flash:
    • ISO setting:
    • White Balance:
    • Exposure:
    • Saturation:
    • Contrast:


Calibration

[Instructions: Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.]

  • Distance between the smart phone cradle and drop =

[Instructions: See worksheet page 6.]


Solutions Used for Calibration [Instructions: See worksheet page 6.]

row 1 cell 1 row 1 cell 2 row 1 cell 3 row 1 cell 4
row 2 cell 1 row 2 cell 2 row 2 cell 3 row 2 cell 4
row 3 cell 1 row 3 cell 2 row 3 cell 3 row 3 cell 4

[Add more rows as needed]


Placing Samples onto the Fluorimeter

  1. [Instructions: Step one, in your OWN words]
  2. [Instructions: Step two, in your own words]
  3. [Instructions: Step three, in your own words]
  4. [Instructions: Step etc., in your own words]


Data Analysis

Representative Images of Samples

[Instructions: Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)]


Image J Values for All Samples


TABLE GOES HERE


Fitting a Straight Line


PCR Results Summary

  • Our positive control PCR result was ____ μg/mL
  • Our negative control PCR result was ____ μg/mL

Observed results

  • Patient _____ :
  • Patient _____ :

Conclusions

  • Patient _____ :
  • Patient _____ :