Template:ASUBME103 s2014 L4: Difference between revisions

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'''The Original Design'''<br>
'''The Original Design'''<br>
(Add image of the full OpenPCR machine here, from the Week 9 exercise. Write a paragraph description for visitors who have no idea what this is)<br>
<!-- Add an image of the full OpenPCR machine here, from the Week 9 exercise. Write a paragraph description for viewers who have no idea what this is) -->




'''Experimenting With the Connections'''<br>
'''Experimenting With the Connections'''<br>
 
<!-- When we unplugged (part 3) from (part 6), the machine ... (did what?). When we unplugged the white wire that connects (part 6) to (part 2), the machine ... (did what?) -->
When we unplugged (part 3) from (part 6), the machine ... (did what? fill in your answer)
 
When we unplugged the white wire that connects (part 6) to (part 2), the machine ... (did what? fill in your answer)




'''Test Run'''
'''Test Run'''
 
<!-- Write the date you first tested the Open PCR machine and your experience(s) with the machine --><br>
(Write the date you first tested Open PCR and your experience(s) with the machine)<br>




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'''PCR Reaction Mix'''
'''PCR Reaction Mix'''
* What is in the PCR reaction mix?
<!-- Make a bulleted list of the components that make up the PCR Reaction Mix -->
 
* Component 1
* Component 2
* Component 3...


'''DNA/ primer mix'''
* What is in the DNA/ primer mix?


'''DNA/ primer Mix'''
<!-- Make a bulleted list of the specific components that make up the DNA/ primer Mix -->
* Component 1
* Component 2
* Component 3...




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'''PCR - The Underlying Technology'''<br>
'''PCR - The Underlying Technology'''<br>
<!-- Add a write-up, essay-style, organized into paragraphs with descriptive headers, based on the Q&A's from Section three of your worksheet). BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the PCR video/ tutorial might be useful. Be sure to '''credit the sources''' if you borrow images. -->


(Add a write-up, essay-style, organized into paragraphs with descriptive headers, based on the Q&A's from Section three of your worksheet)<br>


(BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the PCR video/ tutorial might be useful. Be sure to '''credit the sources''' if you borrow images.)




Revision as of 14:41, 16 August 2014

BME 100 Spring 2014 Home
People
Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
Course Logistics For Instructors
Photos
Wiki Editing Help


OUR TEAM

Name: student
Role(s)
Name: student
Role(s)
Name: student
Role(s)
Name: student
Role(s)
Name: student
Role(s)
Name: student
Role(s)

LAB 1 WRITE-UP

Initial Machine Testing

The Original Design


Experimenting With the Connections


Test Run




Protocols

Thermal Cycler Program


DNA Sample Set-up

row 1 cell 1 row 1 cell 2 row 1 cell 3 row 1 cell 4
row 2 cell 1 row 2 cell 2 row 2 cell 3 row 2 cell 4


DNA Sample Set-up Procedure

  1. Step 1
  2. Step 2
  3. Step 3...


PCR Reaction Mix

  • Component 1
  • Component 2
  • Component 3...


DNA/ primer Mix

  • Component 1
  • Component 2
  • Component 3...




Research and Development

PCR - The Underlying Technology






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