Team kansai result: Difference between revisions
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== <font face="Helvetica" color="#298cda">Erectrophoresis</font> == | == <font face="Helvetica" color="#298cda">Erectrophoresis</font> == | ||
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Formation of Stage + Convex DNA origami was confirmed by erectrophoresis and the result is shown in Fig. 6. | |||
It was confirmed to construct Stage and Convex DNA origami because of the bands of Lane 2 (in red parentheses) and Lane 3 (in orange parentheses) have different electrophoretic mobility compared with Lane 1. | It was confirmed to construct Stage and Convex DNA origami because of the bands of Lane 2 (in red parentheses) and Lane 3 (in orange parentheses) have different electrophoretic mobility compared with Lane 1. | ||
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[[Image:Stage AFM.png|thumb|300px|left|<font face="Helvetica">Fig. 5. AFM images of (a) Stage, (b) Convex DNA origami and (c) Stage + Convex DNA origami.</font>]] | |||
[[Image:result of electrophoresis.png|thumb|430px|right|<font face="Helvetica">Fig. 6. The result of electrophoresis. Lane 1, M13mp18 ssDNA. Lane 2, Stage. Lane 3, Convex DNA origami. Lane 4, Stage + Convex DNA origami. ([[Media:raw data.jpg|Raw data]])</font>]] | |||
== <font face="Helvetica" color="#298cda">Molecular NINJA</font> == | == <font face="Helvetica" color="#298cda">Molecular NINJA</font> == | ||
<!--[[Image:profile of S + C.png|600px]] | <!--[[Image:profile of S + C.png|600px]] | ||
Revision as of 01:11, 29 October 2011
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| Top | Team | Project | Results | Protocols | photos |
Stage
First, We constructed the stage and the result is shown in Fig. 5 (a). As you can see, the stage was successfully constructed. But most stages make complexes by blunt-end π–π stacking interactions. To suppress making complexes, thymine spacers were inserted in staple DNAs which is located at side of the stage.
Convex DNA origami
Next, we tried to construct the convex DNA origami and the result is shown in Fig. 5 (b). Convex DNA origami was successfully constructed.
Stage + Convex DNA origami
It was confirmed that Stage and Convex DNA origami were constructed. Next, we tried to combine them. The result is shown in Fig. 5 (c) and confirmed that Stage was combined with Convex DNA origami which width was observed ~17 nm. At first time, the yield was ~20% and a reason of the low yield is that the mixture was only incubated at room temperature without slowly annealing. After this, Stage + Convex DNA origami was constructed with slowly annealling from 40℃ to room temperature and the yield rose up to ~60%.
Erectrophoresis
Formation of Stage + Convex DNA origami was confirmed by erectrophoresis and the result is shown in Fig. 6. It was confirmed to construct Stage and Convex DNA origami because of the bands of Lane 2 (in red parentheses) and Lane 3 (in orange parentheses) have different electrophoretic mobility compared with Lane 1.
