Team kansai result: Difference between revisions
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== <font face="Helvetica" color="#298cda">Stage</font> == | == <font face="Helvetica" color="#298cda">Stage</font> == | ||
[[Image:Stage AFM.png|thumb|400px|Fig. 6. AFM images of (a) Stage, (b) Convex DNA origami and (c) Stage + Convex DNA origami.]] | [[Image:Stage AFM.png|thumb|400px|<font face="Helvetica">Fig. 6. AFM images of (a) Stage, (b) Convex DNA origami and (c) Stage + Convex DNA origami.</font>]] | ||
<p style="line-height: 200%"> <font face="Helvetica"> | <p style="line-height: 200%"> <font face="Helvetica"> | ||
First, We constructed the stage and the result is shown in Fig. 6 (a). | First, We constructed the stage and the result is shown in Fig. 6 (a). | ||
Revision as of 03:31, 28 October 2011
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| Top | Team | Project | Results | Protocols | photos |
Stage
First, We constructed the stage and the result is shown in Fig. 6 (a). As you can see, the stage was successfully constructed. But most stages make complexes by blunt-end π–π stacking interactions. To suppress making complexes, thymine spacers were inserted in staple DNAs which is located at side of the stage.
Convex DNA origami
Next, we tried to construct the convex DNA origami and the result is shown in Fig. 6 (b). Convex DNA origami was successfully constructed.
Stage + Convex DNA origami
It was confirmed that Stage and Convex DNA origami were constructed. Next, we tried to combine them. The result is shown in Fig. 6 (c) and confirmed that Stage was combined with Convex DNA origami which width was observed ~17 nm. At first time, the yield was ~20% and a reason of the low yield is that the mixture was only incubated at room temperature without slowly annealing. After this, Stage + Convex DNA origami was constructed with slowly annealling from 40℃ to room temperature and the yield rose up to ~60%.
