Talk:Neidhardt EZ Rich Defined: Difference between revisions
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Using glycerol as the standard carbon source seems like a good choice and I have now indicated that on the main page. Nonetheless, some lab members who do not need to fully conform to SBWG standardization may still want to use the media, which is why I was not as yet going to include the sugar by default.--[[User:Bcanton|BC]] 15:07, 7 Jun 2005 (EDT) | Using glycerol as the standard carbon source seems like a good choice and I have now indicated that on the main page. Nonetheless, some lab members who do not need to fully conform to SBWG standardization may still want to use the media, which is why I was not as yet going to include the sugar by default.--[[User:Bcanton|BC]] 15:07, 7 Jun 2005 (EDT) | ||
*'''[[User:Austin J. Che|Austin Che]] 19:29, 13 June 2007 (EDT)''': I must have completely missed these discussions whenever they happened but glycerol isn't really that great a choice as E. coli '''require''' oxygen to grow in glycerol. They cannot grow anaerobically, i.e. ferment glycerol, like they can with glucose due to the balance of NADH for glycolysis/fermentation. Thus I do not think glycerol is a great choice. | |||
Latest revision as of 16:29, 13 June 2007
As per earlier discussions with others in the SBWG, we should likely further standardize this media by using glycerol as the carbon source. --Reshma 14:38, 7 Jun 2005 (EDT)
Using glycerol as the standard carbon source seems like a good choice and I have now indicated that on the main page. Nonetheless, some lab members who do not need to fully conform to SBWG standardization may still want to use the media, which is why I was not as yet going to include the sugar by default.--BC 15:07, 7 Jun 2005 (EDT)
- Austin Che 19:29, 13 June 2007 (EDT): I must have completely missed these discussions whenever they happened but glycerol isn't really that great a choice as E. coli require oxygen to grow in glycerol. They cannot grow anaerobically, i.e. ferment glycerol, like they can with glucose due to the balance of NADH for glycolysis/fermentation. Thus I do not think glycerol is a great choice.
