Talk:Knight:Purification of His-tagged proteins/Denaturing: Difference between revisions
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Latest revision as of 13:42, 24 September 2006
Sample results
Lane order:
- SeeBlue Plus2 Pre-Stained Standard
- Cleared lysate
- Flow through (after column binding)
- Flow through (after column binding, second time)
- Wash 1
- Wash 2
- Eluted protein
- Cleared lysate from cells not expressing a His-tagged protein
- Flow through (after column binding, second time) from cells not expressing a His-tagged protein
- Wash 2 from cells not expressing a His-tagged protein