http://openwetware.org/index.php?title=Talk:Haynes:Gibson_Assembly&feed=atom&action=history 2013-05-23T20:25:56Z Revision history for this page on the wiki MediaWiki 1.13.2 http://openwetware.org/index.php?title=Talk:Haynes:Gibson_Assembly&diff=666372&oldid=prev 2013-01-09T06:39:45Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 06:39, 9 January 2013</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 2:</td> <td colspan="2" class="diff-lineno">Line 2:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''[[User:Karmella Haynes|---Karmella]] 18:14, 5 January 2013 (EST)''': Please add the Gibson reaction to this page (reagents, amounts, incubation, transformation, etc.)</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''[[User:Karmella Haynes|---Karmella]] 18:14, 5 January 2013 (EST)''': Please add the Gibson reaction to this page (reagents, amounts, incubation, transformation, etc.)</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*'''[[User:Karmella Haynes|---Karmella]] 01:39, 9 January 2013 (EST)''': I think this method may not be the best for fragments shorter than 100 bp or so. Endonuclease might chew up short fragments.</ins></div></td></tr> <!-- diff generator: internal 2013-05-23 20:25:56 --> </table>

Karmella Haynes http://openwetware.org/index.php?title=Talk:Haynes:Gibson_Assembly&diff=665583&oldid=prev 2013-01-05T23:14:12Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 23:14, 5 January 2013</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''[[User:Karmella Haynes|---Karmella]] 22:30, 26 November 2012 (EST)''': Vi, Brady and I are going to try combining Gibson-ized PCR products with a XbaI/SpeI cut BioBrick vector. The BioBrick prefix and suffix are a convenient ~20bp long. If this works, we may not have to PCR-amplify the vector as long as the Gibson inserts have BioBrick ends.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''[[User:Karmella Haynes|---Karmella]] 22:30, 26 November 2012 (EST)''': Vi, Brady and I are going to try combining Gibson-ized PCR products with a XbaI/SpeI cut BioBrick vector. The BioBrick prefix and suffix are a convenient ~20bp long. If this works, we may not have to PCR-amplify the vector as long as the Gibson inserts have BioBrick ends.</div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td colspan="2">&nbsp;</td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*'''[[User:Karmella Haynes|---Karmella]] 18:14, 5 January 2013 (EST)''': Please add the Gibson reaction to this page (reagents, amounts, incubation, transformation, etc.)</ins></div></td></tr> <!-- diff generator: internal 2013-05-23 20:25:56 --> </table>

Karmella Haynes http://openwetware.org/index.php?title=Talk:Haynes:Gibson_Assembly&diff=658894&oldid=prev 2012-11-27T03:30:10Z

<p>New page: *'''~~~~''': Vi, Brady and I are going to try combining Gibson-ized PCR products with a XbaI/SpeI cut BioBrick vector. The BioBrick prefix and suffix are a convenient ~20bp long. If this w...</p> <p><b>New page</b></p><div>*'''[[User:Karmella Haynes|---Karmella]] 22:30, 26 November 2012 (EST)''': Vi, Brady and I are going to try combining Gibson-ized PCR products with a XbaI/SpeI cut BioBrick vector. The BioBrick prefix and suffix are a convenient ~20bp long. If this works, we may not have to PCR-amplify the vector as long as the Gibson inserts have BioBrick ends.</div>

Karmella Haynes