Talk:Haynes:Gibson Assembly

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(New page: *'''~~~~''': Vi, Brady and I are going to try combining Gibson-ized PCR products with a XbaI/SpeI cut BioBrick vector. The BioBrick prefix and suffix are a convenient ~20bp long. If this w...)
Current revision (02:39, 9 January 2013) (view source)
 
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*'''[[User:Karmella Haynes|---Karmella]] 22:30, 26 November 2012 (EST)''': Vi, Brady and I are going to try combining Gibson-ized PCR products with a XbaI/SpeI cut BioBrick vector. The BioBrick prefix and suffix are a convenient ~20bp long. If this works, we may not have to PCR-amplify the vector as long as the Gibson inserts have BioBrick ends.
*'''[[User:Karmella Haynes|---Karmella]] 22:30, 26 November 2012 (EST)''': Vi, Brady and I are going to try combining Gibson-ized PCR products with a XbaI/SpeI cut BioBrick vector. The BioBrick prefix and suffix are a convenient ~20bp long. If this works, we may not have to PCR-amplify the vector as long as the Gibson inserts have BioBrick ends.
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*'''[[User:Karmella Haynes|---Karmella]] 18:14, 5 January 2013 (EST)''': Please add the Gibson reaction to this page (reagents, amounts, incubation, transformation, etc.)
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*'''[[User:Karmella Haynes|---Karmella]] 01:39, 9 January 2013 (EST)''': I think this method may not be the best for fragments shorter than 100 bp or so. Endonuclease might chew up short fragments.

Current revision

  • ---Karmella 22:30, 26 November 2012 (EST): Vi, Brady and I are going to try combining Gibson-ized PCR products with a XbaI/SpeI cut BioBrick vector. The BioBrick prefix and suffix are a convenient ~20bp long. If this works, we may not have to PCR-amplify the vector as long as the Gibson inserts have BioBrick ends.
  • ---Karmella 18:14, 5 January 2013 (EST): Please add the Gibson reaction to this page (reagents, amounts, incubation, transformation, etc.)
  • ---Karmella 01:39, 9 January 2013 (EST): I think this method may not be the best for fragments shorter than 100 bp or so. Endonuclease might chew up short fragments.
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