Talk:Endy:Preparing Antibiotic Stocks

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Chloramphenicol
   * Stock concentration - 34mg/ml in 100% Ethanol
   * Aliquots - 1ml
   * Working concentration = 25μ/ml (Stringent), 170μ/ml (relaxed) 

Are stringent and relaxed the wrong way around...?

  • Jason R. Kelly 07:08, 17 January 2008 (CST): I think it's right. Relaxed plasmids are normally higher copy, so you'd expect a higher concentration of antibiotic for them.
  • hugh kingston 10:16, 4 February 2008 (CST): ok. i did not realise stringent and relaxed were types of plasmid, rather i thought the words referred to the strength of selection for the marker.


Can anyone provide some information about temperature inactivation of certain antibiotics. I know that it's OK to add some straight to hot agar, whilst others would be inactivated by anything more than hand-hot, but I don't know which. It may also be possible to autoclave some, which could potentially be useful for preparing stocks, making it possible to avoid fiddley filter-sterilization. I've googled around, but haven't managed to find anything. Someone with more knowledge and experience than me may be able to provide some useful information! Cheers.

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