Talk:Endy:Preparing Antibiotic Stocks: Difference between revisions
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(Asked whether anyone knows about temperature inactivation of antibiotics - for adding to hot agar, or for autoclaving.) |
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*'''[[User:Jason R. Kelly|Jason R. Kelly]] 07:08, 17 January 2008 (CST):''' I think it's right. Relaxed plasmids are normally higher copy, so you'd expect a higher concentration of antibiotic for them. | *'''[[User:Jason R. Kelly|Jason R. Kelly]] 07:08, 17 January 2008 (CST):''' I think it's right. Relaxed plasmids are normally higher copy, so you'd expect a higher concentration of antibiotic for them. | ||
*'''[[User:Hugh Kingston|hugh kingston]] 10:16, 4 February 2008 (CST)''': ok. i did not realise stringent and relaxed were types of plasmid, rather i thought the words referred to the strength of selection for the marker. | *'''[[User:Hugh Kingston|hugh kingston]] 10:16, 4 February 2008 (CST)''': ok. i did not realise stringent and relaxed were types of plasmid, rather i thought the words referred to the strength of selection for the marker. | ||
Can anyone provide some information about temperature inactivation of certain antibiotics. I know that it's OK to add some straight to hot agar, whilst others would be inactivated by anything more than hand-hot, but I don't know which. It may also be possible to autoclave some, which could potentially be useful for preparing stocks, making it possible to avoid fiddley filter-sterilization. I've googled around, but haven't managed to find anything. Someone with more knowledge and experience than me may be able to provide some useful information! Cheers. | |||
Latest revision as of 07:54, 18 June 2013
Chloramphenicol
* Stock concentration - 34mg/ml in 100% Ethanol * Aliquots - 1ml * Working concentration = 25μ/ml (Stringent), 170μ/ml (relaxed)
Are stringent and relaxed the wrong way around...?
- Jason R. Kelly 07:08, 17 January 2008 (CST): I think it's right. Relaxed plasmids are normally higher copy, so you'd expect a higher concentration of antibiotic for them.
- hugh kingston 10:16, 4 February 2008 (CST): ok. i did not realise stringent and relaxed were types of plasmid, rather i thought the words referred to the strength of selection for the marker.
Can anyone provide some information about temperature inactivation of certain antibiotics. I know that it's OK to add some straight to hot agar, whilst others would be inactivated by anything more than hand-hot, but I don't know which. It may also be possible to autoclave some, which could potentially be useful for preparing stocks, making it possible to avoid fiddley filter-sterilization. I've googled around, but haven't managed to find anything. Someone with more knowledge and experience than me may be able to provide some useful information! Cheers.
