Talk:Endy:Preparing Antibiotic Stocks

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(New page: Chloramphenicol * Stock concentration - 34mg/ml in 100% Ethanol * Aliquots - 1ml * Working concentration = 25μ/ml (Stringent), 170μ/ml (relaxed) Are stringent and relaxed...)
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Are stringent and relaxed the wrong way around...?
Are stringent and relaxed the wrong way around...?
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*'''[[User:Jason R. Kelly|Jason R. Kelly]] 07:08, 17 January 2008 (CST):''' I think it's right.  Relaxed plasmids are normally higher copy, so you'd expect a higher concentration of antibiotic for them.

Revision as of 08:08, 17 January 2008

Chloramphenicol
   * Stock concentration - 34mg/ml in 100% Ethanol
   * Aliquots - 1ml
   * Working concentration = 25μ/ml (Stringent), 170μ/ml (relaxed) 

Are stringent and relaxed the wrong way around...?

  • Jason R. Kelly 07:08, 17 January 2008 (CST): I think it's right. Relaxed plasmids are normally higher copy, so you'd expect a higher concentration of antibiotic for them.
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