Talk:Endy:Preparing Antibiotic Stocks

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Current revision (09:54, 18 June 2013) (view source)
(Asked whether anyone knows about temperature inactivation of antibiotics - for adding to hot agar, or for autoclaving.)
 
Line 8: Line 8:
*'''[[User:Jason R. Kelly|Jason R. Kelly]] 07:08, 17 January 2008 (CST):''' I think it's right.  Relaxed plasmids are normally higher copy, so you'd expect a higher concentration of antibiotic for them.
*'''[[User:Jason R. Kelly|Jason R. Kelly]] 07:08, 17 January 2008 (CST):''' I think it's right.  Relaxed plasmids are normally higher copy, so you'd expect a higher concentration of antibiotic for them.
*'''[[User:Hugh Kingston|hugh kingston]] 10:16, 4 February 2008 (CST)''': ok. i did not realise stringent and relaxed were types of plasmid, rather i thought the words referred to the strength of selection for the marker.
*'''[[User:Hugh Kingston|hugh kingston]] 10:16, 4 February 2008 (CST)''': ok. i did not realise stringent and relaxed were types of plasmid, rather i thought the words referred to the strength of selection for the marker.
 +
 +
 +
 +
Can anyone provide some information about temperature inactivation of certain antibiotics.  I know that it's OK to add some straight to hot agar, whilst others would be inactivated by anything more than hand-hot, but I don't know which.  It may also be possible to autoclave some, which could potentially be useful for preparing stocks, making it possible to avoid fiddley filter-sterilization.  I've googled around, but haven't managed to find anything.  Someone with more knowledge and experience than me may be able to provide some useful information!  Cheers.

Current revision

Chloramphenicol
   * Stock concentration - 34mg/ml in 100% Ethanol
   * Aliquots - 1ml
   * Working concentration = 25μ/ml (Stringent), 170μ/ml (relaxed) 

Are stringent and relaxed the wrong way around...?

  • Jason R. Kelly 07:08, 17 January 2008 (CST): I think it's right. Relaxed plasmids are normally higher copy, so you'd expect a higher concentration of antibiotic for them.
  • hugh kingston 10:16, 4 February 2008 (CST): ok. i did not realise stringent and relaxed were types of plasmid, rather i thought the words referred to the strength of selection for the marker.


Can anyone provide some information about temperature inactivation of certain antibiotics. I know that it's OK to add some straight to hot agar, whilst others would be inactivated by anything more than hand-hot, but I don't know which. It may also be possible to autoclave some, which could potentially be useful for preparing stocks, making it possible to avoid fiddley filter-sterilization. I've googled around, but haven't managed to find anything. Someone with more knowledge and experience than me may be able to provide some useful information! Cheers.

Personal tools