Talk:CHE391L/S13/Genome Editing

Benjamin Gilman 12:29, 8 April 2013 (EDT): Currently planned edits:

• Benjamin Gilman 13:23, 8 April 2013 (EDT): Fix typos
• Benjamin Gilman 12:29, 8 April 2013 (EDT): Add a few references, particularly for targeting in different organisms. Move reference tags.
• Benjamin Gilman 12:29, 8 April 2013 (EDT): Finish/add a new figure that shows a simple comparison of ZFN, TALEN, and rGEN target sites and structure of Cas9/sgRNA (and isn't under nature copyright)
• Benjamin Gilman 14:28, 8 April 2013 (EDT): Add mention of 2012 Freiburg iGEM team which biobricked TAL domains, Harvard 2012 zinc finger binding domain search
• Benjamin Gilman 19:29, 8 April 2013 (EDT): Use CASCADE nomenclature for type I CRISPR nuclease complex

• Kevin Baldridge 17:01, 8 April 2013 (EDT):In the CRISPR/Cas9 section, you refer to crRNAs -- I assume this is cis-repressive, but it might but good to write that out with the abbreviation the first time it's mentioned
  • Benjamin Gilman 19:26, 8 April 2013 (EDT): It's a little silly, but crRNA just stands for CRISPR RNA. I'll make a note of it in the text.
• Catherine I. Mortensen 20:23, 9 April 2013 (EDT):I clicked around on the Targetron page but I couldn't find any specifics on their method of integrating DNA. Do you know how it works? I've never heard of integration without endonucleases, seems interesting.