Talk:CHE391L/S13/Genome Editing

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*'''[[User:Kevin Baldridge|Kevin Baldridge]] 17:01, 8 April 2013 (EDT)''':In the CRISPR/Cas9 section, you refer to crRNAs -- I assume this is cis-repressive, but it might but good to write that out with the abbreviation the first time it's mentioned
*'''[[User:Kevin Baldridge|Kevin Baldridge]] 17:01, 8 April 2013 (EDT)''':In the CRISPR/Cas9 section, you refer to crRNAs -- I assume this is cis-repressive, but it might but good to write that out with the abbreviation the first time it's mentioned
**'''[[User:Benjamin Gilman|Benjamin Gilman]] 19:26, 8 April 2013 (EDT)''': It's a little silly, but crRNA just stands for CRISPR RNA.  I'll make a note of it in the text.
**'''[[User:Benjamin Gilman|Benjamin Gilman]] 19:26, 8 April 2013 (EDT)''': It's a little silly, but crRNA just stands for CRISPR RNA.  I'll make a note of it in the text.
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*'''[[User:Catherine I. Mortensen|Catherine I. Mortensen]] 20:23, 9 April 2013 (EDT)''':I clicked around on the Targetron page but I couldn't find any specifics on their method of integrating DNA. Do you know how it works? I've never heard of integration without endonucleases, seems interesting.

Revision as of 19:23, 9 April 2013

Benjamin Gilman 12:29, 8 April 2013 (EDT): Currently planned edits:

  • Benjamin Gilman 13:23, 8 April 2013 (EDT): Fix typos
  • Benjamin Gilman 12:29, 8 April 2013 (EDT): Add a few references, particularly for targeting in different organisms. Move reference tags.
  • Benjamin Gilman 12:29, 8 April 2013 (EDT): Finish/add a new figure that shows a simple comparison of ZFN, TALEN, and rGEN target sites and structure of Cas9/sgRNA (and isn't under nature copyright)
  • Benjamin Gilman 14:28, 8 April 2013 (EDT): Add mention of 2012 Freiburg iGEM team which biobricked TAL domains, Harvard 2012 zinc finger binding domain search
  • Benjamin Gilman 19:29, 8 April 2013 (EDT): Use CASCADE nomenclature for type I CRISPR nuclease complex
  • Kevin Baldridge 17:01, 8 April 2013 (EDT):In the CRISPR/Cas9 section, you refer to crRNAs -- I assume this is cis-repressive, but it might but good to write that out with the abbreviation the first time it's mentioned
    • Benjamin Gilman 19:26, 8 April 2013 (EDT): It's a little silly, but crRNA just stands for CRISPR RNA. I'll make a note of it in the text.
  • Catherine I. Mortensen 20:23, 9 April 2013 (EDT):I clicked around on the Targetron page but I couldn't find any specifics on their method of integrating DNA. Do you know how it works? I've never heard of integration without endonucleases, seems interesting.
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