Talk:CH391L/S2013 Hala Ouzon Jan 23 2013 - Revision history

Ilya J. Finkelstein at 18:59, 30 January 2013

Ilya J. Finkelstein — Wed, 30 Jan 2013 18:59:20 GMT

←Older revision		Revision as of 18:59, 30 January 2013
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	*'''[[User:Tanya_E_Raymond\|Tanya Raymond]] 15:48, 23 January 2013 (EST)''' What are other roles of BRCA2 in humans?		*'''[[User:Tanya_E_Raymond\|Tanya Raymond]] 15:48, 23 January 2013 (EST)''' What are other roles of BRCA2 in humans?
	*'''[[User:Tanya_E_Raymond\|Tanya Raymond]] 15:48, 23 January 2013 (EST)''' Are there other proteins that interact with Rad51 and assist with exchange between Rad51 and RPA at ssDNA?		*'''[[User:Tanya_E_Raymond\|Tanya Raymond]] 15:48, 23 January 2013 (EST)''' Are there other proteins that interact with Rad51 and assist with exchange between Rad51 and RPA at ssDNA?
-	**[[User:Ilya J. Finkelstein]]There are a number of proteins that interact with Rad51 and RPA -- in fact, these days RPA is thought to interact with proteins in ALL replication and repair pathways. I am sure BRCA2 is an important, but incomplete part of the Rad51 loading story. For example, look at Wolf-Deitrich Heyer's recent paper on Rad55-57: http://www.ncbi.nlm.nih.gov/pubmed/22020281	+	**[[User:Ilya J. Finkelstein\|Ilya Finkelstein]] There are a number of proteins that interact with Rad51 and RPA -- in fact, these days RPA is thought to interact with proteins in ALL replication and repair pathways. I am sure BRCA2 is an important, but incomplete part of the Rad51 loading story. For example, look at Wolf-Deitrich Heyer's recent paper on Rad55-57: http://www.ncbi.nlm.nih.gov/pubmed/22020281
	*'''[[User:Logan_R._Myler\|Logan Myler]]''' Both RPA and Rad51 are single-stranded DNA binding proteins homologous to SSB and RecA in E. coli respectively. Without BRCA2, how does E. coli displace SSB(RPA), and why is BRCA2 important for mammalian cells? In general, what is the importance of having both RPA and Rad51 instead of one single-stranded DNA binding protein that can promote homologous recombination?		*'''[[User:Logan_R._Myler\|Logan Myler]]''' Both RPA and Rad51 are single-stranded DNA binding proteins homologous to SSB and RecA in E. coli respectively. Without BRCA2, how does E. coli displace SSB(RPA), and why is BRCA2 important for mammalian cells? In general, what is the importance of having both RPA and Rad51 instead of one single-stranded DNA binding protein that can promote homologous recombination?
		+	**[[User:Ilya J. Finkelstein\|Ilya Finkelstein]] An excellent question. Unlike Rad51, RecA does not bind dsDNA easily -- in fact it only binds ssDNA under physiological conditions. In addition, both the RecBCD resection enzyme and RecFOR enzymes physically interact with RecA to load it onto ssDNA, reducing the need for a BRCA2-like mechanism.
		+

	#REDIRECT [[CH391L/S2013 Hala Ouzon Jan 23 2013]]		#REDIRECT [[CH391L/S2013 Hala Ouzon Jan 23 2013]]

Ilya J. Finkelstein at 18:57, 30 January 2013

Ilya J. Finkelstein — Wed, 30 Jan 2013 18:57:15 GMT

←Older revision		Revision as of 18:57, 30 January 2013
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	*'''[[User:Tanya_E_Raymond\|Tanya Raymond]] 15:48, 23 January 2013 (EST)''' What are other roles of BRCA2 in humans?		*'''[[User:Tanya_E_Raymond\|Tanya Raymond]] 15:48, 23 January 2013 (EST)''' What are other roles of BRCA2 in humans?
	*'''[[User:Tanya_E_Raymond\|Tanya Raymond]] 15:48, 23 January 2013 (EST)''' Are there other proteins that interact with Rad51 and assist with exchange between Rad51 and RPA at ssDNA?		*'''[[User:Tanya_E_Raymond\|Tanya Raymond]] 15:48, 23 January 2013 (EST)''' Are there other proteins that interact with Rad51 and assist with exchange between Rad51 and RPA at ssDNA?
-	**There are a number of proteins that interact with Rad51 and RPA -- in fact, these days RPA is thought to interact with proteins in ALL replication and repair pathways. I am sure BRCA2 is an important, but incomplete part of the Rad51 loading story. For example, look at Wolf-Deitrich Heyer's recent paper on Rad55-57: http://www.ncbi.nlm.nih.gov/pubmed/22020281	+	**[[User:Ilya J. Finkelstein]]There are a number of proteins that interact with Rad51 and RPA -- in fact, these days RPA is thought to interact with proteins in ALL replication and repair pathways. I am sure BRCA2 is an important, but incomplete part of the Rad51 loading story. For example, look at Wolf-Deitrich Heyer's recent paper on Rad55-57: http://www.ncbi.nlm.nih.gov/pubmed/22020281
	*'''[[User:Logan_R._Myler\|Logan Myler]]''' Both RPA and Rad51 are single-stranded DNA binding proteins homologous to SSB and RecA in E. coli respectively. Without BRCA2, how does E. coli displace SSB(RPA), and why is BRCA2 important for mammalian cells? In general, what is the importance of having both RPA and Rad51 instead of one single-stranded DNA binding protein that can promote homologous recombination?		*'''[[User:Logan_R._Myler\|Logan Myler]]''' Both RPA and Rad51 are single-stranded DNA binding proteins homologous to SSB and RecA in E. coli respectively. Without BRCA2, how does E. coli displace SSB(RPA), and why is BRCA2 important for mammalian cells? In general, what is the importance of having both RPA and Rad51 instead of one single-stranded DNA binding protein that can promote homologous recombination?

	#REDIRECT [[CH391L/S2013 Hala Ouzon Jan 23 2013]]		#REDIRECT [[CH391L/S2013 Hala Ouzon Jan 23 2013]]

Ilya J. Finkelstein at 18:56, 30 January 2013

Ilya J. Finkelstein — Wed, 30 Jan 2013 18:56:15 GMT

←Older revision		Revision as of 18:56, 30 January 2013
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	*'''[[User:Tanya_E_Raymond\|Tanya Raymond]] 15:48, 23 January 2013 (EST)''' What are other roles of BRCA2 in humans?		*'''[[User:Tanya_E_Raymond\|Tanya Raymond]] 15:48, 23 January 2013 (EST)''' What are other roles of BRCA2 in humans?
	*'''[[User:Tanya_E_Raymond\|Tanya Raymond]] 15:48, 23 January 2013 (EST)''' Are there other proteins that interact with Rad51 and assist with exchange between Rad51 and RPA at ssDNA?		*'''[[User:Tanya_E_Raymond\|Tanya Raymond]] 15:48, 23 January 2013 (EST)''' Are there other proteins that interact with Rad51 and assist with exchange between Rad51 and RPA at ssDNA?
		+	**There are a number of proteins that interact with Rad51 and RPA -- in fact, these days RPA is thought to interact with proteins in ALL replication and repair pathways. I am sure BRCA2 is an important, but incomplete part of the Rad51 loading story. For example, look at Wolf-Deitrich Heyer's recent paper on Rad55-57: http://www.ncbi.nlm.nih.gov/pubmed/22020281
	*'''[[User:Logan_R._Myler\|Logan Myler]]''' Both RPA and Rad51 are single-stranded DNA binding proteins homologous to SSB and RecA in E. coli respectively. Without BRCA2, how does E. coli displace SSB(RPA), and why is BRCA2 important for mammalian cells? In general, what is the importance of having both RPA and Rad51 instead of one single-stranded DNA binding protein that can promote homologous recombination?		*'''[[User:Logan_R._Myler\|Logan Myler]]''' Both RPA and Rad51 are single-stranded DNA binding proteins homologous to SSB and RecA in E. coli respectively. Without BRCA2, how does E. coli displace SSB(RPA), and why is BRCA2 important for mammalian cells? In general, what is the importance of having both RPA and Rad51 instead of one single-stranded DNA binding protein that can promote homologous recombination?

	#REDIRECT [[CH391L/S2013 Hala Ouzon Jan 23 2013]]		#REDIRECT [[CH391L/S2013 Hala Ouzon Jan 23 2013]]

Logan R. Myler at 19:56, 29 January 2013

Logan R. Myler — Tue, 29 Jan 2013 19:56:00 GMT

←Older revision		Revision as of 19:56, 29 January 2013
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	*'''[[User:Tanya_E_Raymond\|Tanya Raymond]] 15:48, 23 January 2013 (EST)''' What are other roles of BRCA2 in humans?		*'''[[User:Tanya_E_Raymond\|Tanya Raymond]] 15:48, 23 January 2013 (EST)''' What are other roles of BRCA2 in humans?
	*'''[[User:Tanya_E_Raymond\|Tanya Raymond]] 15:48, 23 January 2013 (EST)''' Are there other proteins that interact with Rad51 and assist with exchange between Rad51 and RPA at ssDNA?		*'''[[User:Tanya_E_Raymond\|Tanya Raymond]] 15:48, 23 January 2013 (EST)''' Are there other proteins that interact with Rad51 and assist with exchange between Rad51 and RPA at ssDNA?
-	*'''[[User:Logan_R._Myler\|Logan Myler]]''' Both RPA and Rad51 are single-stranded DNA binding proteins homologous to SSB and RecA in E. ~~Coli~~ respectively. Without BRCA2, how does E. coli displace SSB(RPA), and why is BRCA2 important for mammalian cells? In general, what is the importance of having both RPA and Rad51 instead of one single-stranded DNA binding protein that can promote homologous recombination?	+	*'''[[User:Logan_R._Myler\|Logan Myler]]''' Both RPA and Rad51 are single-stranded DNA binding proteins homologous to SSB and RecA in E. coli respectively. Without BRCA2, how does E. coli displace SSB(RPA), and why is BRCA2 important for mammalian cells? In general, what is the importance of having both RPA and Rad51 instead of one single-stranded DNA binding protein that can promote homologous recombination?

	#REDIRECT [[CH391L/S2013 Hala Ouzon Jan 23 2013]]		#REDIRECT [[CH391L/S2013 Hala Ouzon Jan 23 2013]]

Logan R. Myler at 19:54, 29 January 2013

Logan R. Myler — Tue, 29 Jan 2013 19:54:06 GMT

←Older revision		Revision as of 19:54, 29 January 2013
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	*'''[[User:Tanya_E_Raymond\|Tanya Raymond]] 15:48, 23 January 2013 (EST)''' What are other roles of BRCA2 in humans?		*'''[[User:Tanya_E_Raymond\|Tanya Raymond]] 15:48, 23 January 2013 (EST)''' What are other roles of BRCA2 in humans?
	*'''[[User:Tanya_E_Raymond\|Tanya Raymond]] 15:48, 23 January 2013 (EST)''' Are there other proteins that interact with Rad51 and assist with exchange between Rad51 and RPA at ssDNA?		*'''[[User:Tanya_E_Raymond\|Tanya Raymond]] 15:48, 23 January 2013 (EST)''' Are there other proteins that interact with Rad51 and assist with exchange between Rad51 and RPA at ssDNA?
-		+	*'''[[User:Logan_R._Myler\|Logan Myler]]''' Both RPA and Rad51 are single-stranded DNA binding proteins homologous to SSB and RecA in E. Coli respectively. Without BRCA2, how does E. coli displace SSB(RPA), and why is BRCA2 important for mammalian cells? In general, what is the importance of having both RPA and Rad51 instead of one single-stranded DNA binding protein that can promote homologous recombination?

	#REDIRECT [[CH391L/S2013 Hala Ouzon Jan 23 2013]]		#REDIRECT [[CH391L/S2013 Hala Ouzon Jan 23 2013]]

Tanya E Raymond at 22:56, 23 January 2013

Tanya E Raymond — Wed, 23 Jan 2013 22:56:22 GMT

←Older revision		Revision as of 22:56, 23 January 2013
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		+	*'''[[User:Tanya_E_Raymond\|Tanya Raymond]] 15:48, 23 January 2013 (EST)''' What are other roles of BRCA2 in humans?
		+	*'''[[User:Tanya_E_Raymond\|Tanya Raymond]] 15:48, 23 January 2013 (EST)''' Are there other proteins that interact with Rad51 and assist with exchange between Rad51 and RPA at ssDNA?
		+
		+
	#REDIRECT [[CH391L/S2013 Hala Ouzon Jan 23 2013]]		#REDIRECT [[CH391L/S2013 Hala Ouzon Jan 23 2013]]

Hala Ouzon-Shubeita: Talk:CH391L/S2013 Hala Ouzon Jan 23 2013 moved to CH391L/S2013 Hala Ouzon Jan 23 2013

Hala Ouzon-Shubeita — Wed, 23 Jan 2013 06:31:35 GMT

Talk:CH391L/S2013 Hala Ouzon Jan 23 2013 moved to CH391L/S2013 Hala Ouzon Jan 23 2013

New page

#REDIRECT [[CH391L/S2013 Hala Ouzon Jan 23 2013]]

←Older revision		Revision as of 18:59, 30 January 2013
Line 1:		Line 1:
	*'''[[User:Tanya_E_Raymond\|Tanya Raymond]] 15:48, 23 January 2013 (EST)''' What are other roles of BRCA2 in humans?		*'''[[User:Tanya_E_Raymond\|Tanya Raymond]] 15:48, 23 January 2013 (EST)''' What are other roles of BRCA2 in humans?
	*'''[[User:Tanya_E_Raymond\|Tanya Raymond]] 15:48, 23 January 2013 (EST)''' Are there other proteins that interact with Rad51 and assist with exchange between Rad51 and RPA at ssDNA?		*'''[[User:Tanya_E_Raymond\|Tanya Raymond]] 15:48, 23 January 2013 (EST)''' Are there other proteins that interact with Rad51 and assist with exchange between Rad51 and RPA at ssDNA?
-	**[[User:Ilya J. Finkelstein]]There are a number of proteins that interact with Rad51 and RPA -- in fact, these days RPA is thought to interact with proteins in ALL replication and repair pathways. I am sure BRCA2 is an important, but incomplete part of the Rad51 loading story. For example, look at Wolf-Deitrich Heyer's recent paper on Rad55-57: http://www.ncbi.nlm.nih.gov/pubmed/22020281	+	**[[User:Ilya J. Finkelstein\|Ilya Finkelstein]] There are a number of proteins that interact with Rad51 and RPA -- in fact, these days RPA is thought to interact with proteins in ALL replication and repair pathways. I am sure BRCA2 is an important, but incomplete part of the Rad51 loading story. For example, look at Wolf-Deitrich Heyer's recent paper on Rad55-57: http://www.ncbi.nlm.nih.gov/pubmed/22020281
	*'''[[User:Logan_R._Myler\|Logan Myler]]''' Both RPA and Rad51 are single-stranded DNA binding proteins homologous to SSB and RecA in E. coli respectively. Without BRCA2, how does E. coli displace SSB(RPA), and why is BRCA2 important for mammalian cells? In general, what is the importance of having both RPA and Rad51 instead of one single-stranded DNA binding protein that can promote homologous recombination?		*'''[[User:Logan_R._Myler\|Logan Myler]]''' Both RPA and Rad51 are single-stranded DNA binding proteins homologous to SSB and RecA in E. coli respectively. Without BRCA2, how does E. coli displace SSB(RPA), and why is BRCA2 important for mammalian cells? In general, what is the importance of having both RPA and Rad51 instead of one single-stranded DNA binding protein that can promote homologous recombination?
		+	**[[User:Ilya J. Finkelstein\|Ilya Finkelstein]] An excellent question. Unlike Rad51, RecA does not bind dsDNA easily -- in fact it only binds ssDNA under physiological conditions. In addition, both the RecBCD resection enzyme and RecFOR enzymes physically interact with RecA to load it onto ssDNA, reducing the need for a BRCA2-like mechanism.
		+

	#REDIRECT [[CH391L/S2013 Hala Ouzon Jan 23 2013]]		#REDIRECT [[CH391L/S2013 Hala Ouzon Jan 23 2013]]