Talk:CH391L/S13/Riboswitches

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*'''[[User:Catherine I. Mortensen|Catherine I. Mortensen]] 13:45, 25 February 2013 (EST)''': How practical is DNT detecting E. Coli in airports and other public areas considering the time it takes for E. Coli to start producing fluorescent protein?
*'''[[User:Catherine I. Mortensen|Catherine I. Mortensen]] 13:45, 25 February 2013 (EST)''': How practical is DNT detecting E. Coli in airports and other public areas considering the time it takes for E. Coli to start producing fluorescent protein?
*'''[[User:Kevin Baldridge|Kevin Baldridge]] 17:08, 25 February 2013 (EST)''':Are there any known examples of a riboswitch with an IRES (Internal Ribosome Entry Site, an alternate way to initiate translation that doesn't use the Shine Dalgarno sequence) expression platform? If not, that might be something we could look into for iGEM, directed evolution or SELEX to design such a system.
*'''[[User:Kevin Baldridge|Kevin Baldridge]] 17:08, 25 February 2013 (EST)''':Are there any known examples of a riboswitch with an IRES (Internal Ribosome Entry Site, an alternate way to initiate translation that doesn't use the Shine Dalgarno sequence) expression platform? If not, that might be something we could look into for iGEM, directed evolution or SELEX to design such a system.
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*'''[[User:Gabriel Wu|Gabriel Wu]]''': Connecting with Max's topic, directed evolution strategies are very relevant in  developing riboswitches, especially for aptamer design. Might be nice to discuss the connection between the two topics.

Revision as of 17:17, 25 February 2013

  • Catherine I. Mortensen 13:45, 25 February 2013 (EST): How practical is DNT detecting E. Coli in airports and other public areas considering the time it takes for E. Coli to start producing fluorescent protein?
  • Kevin Baldridge 17:08, 25 February 2013 (EST):Are there any known examples of a riboswitch with an IRES (Internal Ribosome Entry Site, an alternate way to initiate translation that doesn't use the Shine Dalgarno sequence) expression platform? If not, that might be something we could look into for iGEM, directed evolution or SELEX to design such a system.
  • Gabriel Wu: Connecting with Max's topic, directed evolution strategies are very relevant in developing riboswitches, especially for aptamer design. Might be nice to discuss the connection between the two topics.
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