Talk:CH391L/S13/QuorumSensing - Revision history

http://openwetware.org/index.php?title=Talk:CH391L/S13/QuorumSensing&feed=atom&action=history Talk:CH391L/S13/QuorumSensing - Revision history 2013-05-23T23:40:12Z Revision history for this page on the wiki MediaWiki 1.13.2 http://openwetware.org/index.php?title=Talk:CH391L/S13/QuorumSensing&diff=682327&oldid=prev Yunle Huang at 19:18, 10 March 2013 2013-03-10T19:18:07Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 19:18, 10 March 2013</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 9:</td> <td colspan="2" class="diff-lineno">Line 9:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>***'''[[User:Catherine I. Mortensen|Catherine I. Mortensen]] 00:13, 10 March 2013 (EST)''':Agreed, that'd be a useful topic</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>***'''[[User:Catherine I. Mortensen|Catherine I. Mortensen]] 00:13, 10 March 2013 (EST)''':Agreed, that'd be a useful topic</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''[[User:Aurko Dasgupta|Aurko Dasgupta]] 19:17, 7 March 2013 (EST)''': Isn't H<sub>2</sub>O<sub>2</sub> toxic to all sorts of creatures? How does the host system cope with a toxic communication method, or are concentrations used just too low? Does it have a fast catalase response in case it gets swamped with signal?</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''[[User:Aurko Dasgupta|Aurko Dasgupta]] 19:17, 7 March 2013 (EST)''': Isn't H<sub>2</sub>O<sub>2</sub> toxic to all sorts of creatures? How does the host system cope with a toxic communication method, or are concentrations used just too low? Does it have a fast catalase response in case it gets swamped with signal?</div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">**'''[[User:Yunle Huang|Yunle Huang]] 15:18, 10 March 2013 (EDT)''':The article says that low levels of H<sub>2</sub>O<sub>2</sub> are produced, so the concentrations are probably not high enough for it to be toxic.</ins></div></td></tr>  </table>

Yunle Huang http://openwetware.org/index.php?title=Talk:CH391L/S13/QuorumSensing&diff=682228&oldid=prev Catherine I. Mortensen at 05:13, 10 March 2013 2013-03-10T05:13:49Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 05:13, 10 March 2013</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 7:</td> <td colspan="2" class="diff-lineno">Line 7:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**'''[[User:Neil R Gottel|Neil R Gottel]] 19:29, 7 March 2013 (EST)''':With regards to your second question, removing the bacterium's biofilm abilities would certainly reduce it's ability to survive within the body. This is sorta unrelated, but I just came across this paper showing how to [http://www.pnas.org/content/104/27/11197.full use phage to break apart biofilms]. Simply have the phage carry a gene for an enzyme that targets the main structural support of the film, so that infected cells produce large quantities of it. When the cell lyses, the enzymes are released, and expose more bacteria to the phage. Maybe my next topic for the class should be on the role of phage in synthetic biology...</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**'''[[User:Neil R Gottel|Neil R Gottel]] 19:29, 7 March 2013 (EST)''':With regards to your second question, removing the bacterium's biofilm abilities would certainly reduce it's ability to survive within the body. This is sorta unrelated, but I just came across this paper showing how to [http://www.pnas.org/content/104/27/11197.full use phage to break apart biofilms]. Simply have the phage carry a gene for an enzyme that targets the main structural support of the film, so that infected cells produce large quantities of it. When the cell lyses, the enzymes are released, and expose more bacteria to the phage. Maybe my next topic for the class should be on the role of phage in synthetic biology...</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>***'''[[User:Benjamin Gilman|Benjamin Gilman]] 23:29, 7 March 2013 (EST)''': I'll second the phage idea...</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>***'''[[User:Benjamin Gilman|Benjamin Gilman]] 23:29, 7 March 2013 (EST)''': I'll second the phage idea...</div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">***'''[[User:Catherine I. Mortensen|Catherine I. Mortensen]] 00:13, 10 March 2013 (EST)''':Agreed, that'd be a useful topic</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''[[User:Aurko Dasgupta|Aurko Dasgupta]] 19:17, 7 March 2013 (EST)''': Isn't H<sub>2</sub>O<sub>2</sub> toxic to all sorts of creatures? How does the host system cope with a toxic communication method, or are concentrations used just too low? Does it have a fast catalase response in case it gets swamped with signal?</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''[[User:Aurko Dasgupta|Aurko Dasgupta]] 19:17, 7 March 2013 (EST)''': Isn't H<sub>2</sub>O<sub>2</sub> toxic to all sorts of creatures? How does the host system cope with a toxic communication method, or are concentrations used just too low? Does it have a fast catalase response in case it gets swamped with signal?</div></td></tr>   </table>

Catherine I. Mortensen http://openwetware.org/index.php?title=Talk:CH391L/S13/QuorumSensing&diff=682227&oldid=prev Catherine I. Mortensen at 05:10, 10 March 2013 2013-03-10T05:10:01Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 05:10, 10 March 2013</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 3:</td> <td colspan="2" class="diff-lineno">Line 3:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**'''[[User:Neil R Gottel|Neil R Gottel]] 19:29, 7 March 2013 (EST)''':You're refering to those wild strains of P. aeruginosa that were isolated from tarballs, right? I think P. aeruginosa is dangerous only if you have a compromised immune system, or something like cystic fibrosis. I suspect the P. aeurginosa that are living in tarballs probably aren't the same kind that would be infecting humans. The thing with oil spills is that they're naturally occurring (at very low levels, compared to something like Deepwater Horizon), so there's bacteria in the wild that derive their energy at least partially from hydrocarbons.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**'''[[User:Neil R Gottel|Neil R Gottel]] 19:29, 7 March 2013 (EST)''':You're refering to those wild strains of P. aeruginosa that were isolated from tarballs, right? I think P. aeruginosa is dangerous only if you have a compromised immune system, or something like cystic fibrosis. I suspect the P. aeurginosa that are living in tarballs probably aren't the same kind that would be infecting humans. The thing with oil spills is that they're naturally occurring (at very low levels, compared to something like Deepwater Horizon), so there's bacteria in the wild that derive their energy at least partially from hydrocarbons.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>***'''[[User:Benjamin Gilman|Benjamin Gilman]] 23:28, 7 March 2013 (EST)''': Pseudomonas aeruginosa is found pretty much everywhere thanks to the fact that it likes a huge range of surfaces and can grow in hypoxic conditions, so there are a ton of strains out there.  It's a health problem not because it's so infectious, but because you're constantly exposed to it anyway and it's hard to get rid of with antibiotics.  Even if the same strains were used for oil cleanup, I doubt you'd see more cases of it popping up.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>***'''[[User:Benjamin Gilman|Benjamin Gilman]] 23:28, 7 March 2013 (EST)''': Pseudomonas aeruginosa is found pretty much everywhere thanks to the fact that it likes a huge range of surfaces and can grow in hypoxic conditions, so there are a ton of strains out there.  It's a health problem not because it's so infectious, but because you're constantly exposed to it anyway and it's hard to get rid of with antibiotics.  Even if the same strains were used for oil cleanup, I doubt you'd see more cases of it popping up.</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>***'''[[User:Catherine I. Mortensen|Catherine I. Mortensen]] 00:08, 10 March 2013 (EST)''': So I assume their biofilm is the reason why antibiotics are mostly infective<del class="diffchange diffchange-inline">?</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>***'''[[User:Catherine I. Mortensen|Catherine I. Mortensen]] 00:08, 10 March 2013 (EST)''': So I assume their biofilm is the reason why antibiotics are mostly infective</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**'''[[User:Neil R Gottel|Neil R Gottel]] 19:29, 7 March 2013 (EST)''':With regards to your second question, removing the bacterium's biofilm abilities would certainly reduce it's ability to survive within the body. This is sorta unrelated, but I just came across this paper showing how to [http://www.pnas.org/content/104/27/11197.full use phage to break apart biofilms]. Simply have the phage carry a gene for an enzyme that targets the main structural support of the film, so that infected cells produce large quantities of it. When the cell lyses, the enzymes are released, and expose more bacteria to the phage. Maybe my next topic for the class should be on the role of phage in synthetic biology...</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**'''[[User:Neil R Gottel|Neil R Gottel]] 19:29, 7 March 2013 (EST)''':With regards to your second question, removing the bacterium's biofilm abilities would certainly reduce it's ability to survive within the body. This is sorta unrelated, but I just came across this paper showing how to [http://www.pnas.org/content/104/27/11197.full use phage to break apart biofilms]. Simply have the phage carry a gene for an enzyme that targets the main structural support of the film, so that infected cells produce large quantities of it. When the cell lyses, the enzymes are released, and expose more bacteria to the phage. Maybe my next topic for the class should be on the role of phage in synthetic biology...</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>***'''[[User:Benjamin Gilman|Benjamin Gilman]] 23:29, 7 March 2013 (EST)''': I'll second the phage idea...</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>***'''[[User:Benjamin Gilman|Benjamin Gilman]] 23:29, 7 March 2013 (EST)''': I'll second the phage idea...</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''[[User:Aurko Dasgupta|Aurko Dasgupta]] 19:17, 7 March 2013 (EST)''': Isn't H<sub>2</sub>O<sub>2</sub> toxic to all sorts of creatures? How does the host system cope with a toxic communication method, or are concentrations used just too low? Does it have a fast catalase response in case it gets swamped with signal?</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''[[User:Aurko Dasgupta|Aurko Dasgupta]] 19:17, 7 March 2013 (EST)''': Isn't H<sub>2</sub>O<sub>2</sub> toxic to all sorts of creatures? How does the host system cope with a toxic communication method, or are concentrations used just too low? Does it have a fast catalase response in case it gets swamped with signal?</div></td></tr>  </table>

Catherine I. Mortensen http://openwetware.org/index.php?title=Talk:CH391L/S13/QuorumSensing&diff=682226&oldid=prev Catherine I. Mortensen at 05:09, 10 March 2013 2013-03-10T05:09:25Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 05:09, 10 March 2013</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 3:</td> <td colspan="2" class="diff-lineno">Line 3:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**'''[[User:Neil R Gottel|Neil R Gottel]] 19:29, 7 March 2013 (EST)''':You're refering to those wild strains of P. aeruginosa that were isolated from tarballs, right? I think P. aeruginosa is dangerous only if you have a compromised immune system, or something like cystic fibrosis. I suspect the P. aeurginosa that are living in tarballs probably aren't the same kind that would be infecting humans. The thing with oil spills is that they're naturally occurring (at very low levels, compared to something like Deepwater Horizon), so there's bacteria in the wild that derive their energy at least partially from hydrocarbons.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**'''[[User:Neil R Gottel|Neil R Gottel]] 19:29, 7 March 2013 (EST)''':You're refering to those wild strains of P. aeruginosa that were isolated from tarballs, right? I think P. aeruginosa is dangerous only if you have a compromised immune system, or something like cystic fibrosis. I suspect the P. aeurginosa that are living in tarballs probably aren't the same kind that would be infecting humans. The thing with oil spills is that they're naturally occurring (at very low levels, compared to something like Deepwater Horizon), so there's bacteria in the wild that derive their energy at least partially from hydrocarbons.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>***'''[[User:Benjamin Gilman|Benjamin Gilman]] 23:28, 7 March 2013 (EST)''': Pseudomonas aeruginosa is found pretty much everywhere thanks to the fact that it likes a huge range of surfaces and can grow in hypoxic conditions, so there are a ton of strains out there.  It's a health problem not because it's so infectious, but because you're constantly exposed to it anyway and it's hard to get rid of with antibiotics.  Even if the same strains were used for oil cleanup, I doubt you'd see more cases of it popping up.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>***'''[[User:Benjamin Gilman|Benjamin Gilman]] 23:28, 7 March 2013 (EST)''': Pseudomonas aeruginosa is found pretty much everywhere thanks to the fact that it likes a huge range of surfaces and can grow in hypoxic conditions, so there are a ton of strains out there.  It's a health problem not because it's so infectious, but because you're constantly exposed to it anyway and it's hard to get rid of with antibiotics.  Even if the same strains were used for oil cleanup, I doubt you'd see more cases of it popping up.</div></td></tr> <tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>***'''[[User:Catherine I. Mortensen|Catherine I. Mortensen]] 00:08, 10 March 2013 (EST)''':<del class="diffchange diffchange-inline">Does </del>their biofilm <del class="diffchange diffchange-inline">have anything to do with </del>the <del class="diffchange diffchange-inline">fact that </del>antibiotics are mostly infective?</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>***'''[[User:Catherine I. Mortensen|Catherine I. Mortensen]] 00:08, 10 March 2013 (EST)''': <ins class="diffchange diffchange-inline">So I assume </ins>their biofilm <ins class="diffchange diffchange-inline">is </ins>the <ins class="diffchange diffchange-inline">reason why </ins>antibiotics are mostly infective?</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**'''[[User:Neil R Gottel|Neil R Gottel]] 19:29, 7 March 2013 (EST)''':With regards to your second question, removing the bacterium's biofilm abilities would certainly reduce it's ability to survive within the body. This is sorta unrelated, but I just came across this paper showing how to [http://www.pnas.org/content/104/27/11197.full use phage to break apart biofilms]. Simply have the phage carry a gene for an enzyme that targets the main structural support of the film, so that infected cells produce large quantities of it. When the cell lyses, the enzymes are released, and expose more bacteria to the phage. Maybe my next topic for the class should be on the role of phage in synthetic biology...</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**'''[[User:Neil R Gottel|Neil R Gottel]] 19:29, 7 March 2013 (EST)''':With regards to your second question, removing the bacterium's biofilm abilities would certainly reduce it's ability to survive within the body. This is sorta unrelated, but I just came across this paper showing how to [http://www.pnas.org/content/104/27/11197.full use phage to break apart biofilms]. Simply have the phage carry a gene for an enzyme that targets the main structural support of the film, so that infected cells produce large quantities of it. When the cell lyses, the enzymes are released, and expose more bacteria to the phage. Maybe my next topic for the class should be on the role of phage in synthetic biology...</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>***'''[[User:Benjamin Gilman|Benjamin Gilman]] 23:29, 7 March 2013 (EST)''': I'll second the phage idea...</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>***'''[[User:Benjamin Gilman|Benjamin Gilman]] 23:29, 7 March 2013 (EST)''': I'll second the phage idea...</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''[[User:Aurko Dasgupta|Aurko Dasgupta]] 19:17, 7 March 2013 (EST)''': Isn't H<sub>2</sub>O<sub>2</sub> toxic to all sorts of creatures? How does the host system cope with a toxic communication method, or are concentrations used just too low? Does it have a fast catalase response in case it gets swamped with signal?</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''[[User:Aurko Dasgupta|Aurko Dasgupta]] 19:17, 7 March 2013 (EST)''': Isn't H<sub>2</sub>O<sub>2</sub> toxic to all sorts of creatures? How does the host system cope with a toxic communication method, or are concentrations used just too low? Does it have a fast catalase response in case it gets swamped with signal?</div></td></tr>  </table>

Catherine I. Mortensen http://openwetware.org/index.php?title=Talk:CH391L/S13/QuorumSensing&diff=682225&oldid=prev Catherine I. Mortensen at 05:08, 10 March 2013 2013-03-10T05:08:09Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 05:08, 10 March 2013</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 3:</td> <td colspan="2" class="diff-lineno">Line 3:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**'''[[User:Neil R Gottel|Neil R Gottel]] 19:29, 7 March 2013 (EST)''':You're refering to those wild strains of P. aeruginosa that were isolated from tarballs, right? I think P. aeruginosa is dangerous only if you have a compromised immune system, or something like cystic fibrosis. I suspect the P. aeurginosa that are living in tarballs probably aren't the same kind that would be infecting humans. The thing with oil spills is that they're naturally occurring (at very low levels, compared to something like Deepwater Horizon), so there's bacteria in the wild that derive their energy at least partially from hydrocarbons.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**'''[[User:Neil R Gottel|Neil R Gottel]] 19:29, 7 March 2013 (EST)''':You're refering to those wild strains of P. aeruginosa that were isolated from tarballs, right? I think P. aeruginosa is dangerous only if you have a compromised immune system, or something like cystic fibrosis. I suspect the P. aeurginosa that are living in tarballs probably aren't the same kind that would be infecting humans. The thing with oil spills is that they're naturally occurring (at very low levels, compared to something like Deepwater Horizon), so there's bacteria in the wild that derive their energy at least partially from hydrocarbons.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>***'''[[User:Benjamin Gilman|Benjamin Gilman]] 23:28, 7 March 2013 (EST)''': Pseudomonas aeruginosa is found pretty much everywhere thanks to the fact that it likes a huge range of surfaces and can grow in hypoxic conditions, so there are a ton of strains out there.  It's a health problem not because it's so infectious, but because you're constantly exposed to it anyway and it's hard to get rid of with antibiotics.  Even if the same strains were used for oil cleanup, I doubt you'd see more cases of it popping up.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>***'''[[User:Benjamin Gilman|Benjamin Gilman]] 23:28, 7 March 2013 (EST)''': Pseudomonas aeruginosa is found pretty much everywhere thanks to the fact that it likes a huge range of surfaces and can grow in hypoxic conditions, so there are a ton of strains out there.  It's a health problem not because it's so infectious, but because you're constantly exposed to it anyway and it's hard to get rid of with antibiotics.  Even if the same strains were used for oil cleanup, I doubt you'd see more cases of it popping up.</div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">***'''[[User:Catherine I. Mortensen|Catherine I. Mortensen]] 00:08, 10 March 2013 (EST)''':Does their biofilm have anything to do with the fact that antibiotics are mostly infective?</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**'''[[User:Neil R Gottel|Neil R Gottel]] 19:29, 7 March 2013 (EST)''':With regards to your second question, removing the bacterium's biofilm abilities would certainly reduce it's ability to survive within the body. This is sorta unrelated, but I just came across this paper showing how to [http://www.pnas.org/content/104/27/11197.full use phage to break apart biofilms]. Simply have the phage carry a gene for an enzyme that targets the main structural support of the film, so that infected cells produce large quantities of it. When the cell lyses, the enzymes are released, and expose more bacteria to the phage. Maybe my next topic for the class should be on the role of phage in synthetic biology...</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**'''[[User:Neil R Gottel|Neil R Gottel]] 19:29, 7 March 2013 (EST)''':With regards to your second question, removing the bacterium's biofilm abilities would certainly reduce it's ability to survive within the body. This is sorta unrelated, but I just came across this paper showing how to [http://www.pnas.org/content/104/27/11197.full use phage to break apart biofilms]. Simply have the phage carry a gene for an enzyme that targets the main structural support of the film, so that infected cells produce large quantities of it. When the cell lyses, the enzymes are released, and expose more bacteria to the phage. Maybe my next topic for the class should be on the role of phage in synthetic biology...</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>***'''[[User:Benjamin Gilman|Benjamin Gilman]] 23:29, 7 March 2013 (EST)''': I'll second the phage idea...</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>***'''[[User:Benjamin Gilman|Benjamin Gilman]] 23:29, 7 March 2013 (EST)''': I'll second the phage idea...</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''[[User:Aurko Dasgupta|Aurko Dasgupta]] 19:17, 7 March 2013 (EST)''': Isn't H<sub>2</sub>O<sub>2</sub> toxic to all sorts of creatures? How does the host system cope with a toxic communication method, or are concentrations used just too low? Does it have a fast catalase response in case it gets swamped with signal?</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''[[User:Aurko Dasgupta|Aurko Dasgupta]] 19:17, 7 March 2013 (EST)''': Isn't H<sub>2</sub>O<sub>2</sub> toxic to all sorts of creatures? How does the host system cope with a toxic communication method, or are concentrations used just too low? Does it have a fast catalase response in case it gets swamped with signal?</div></td></tr>  </table>

Catherine I. Mortensen http://openwetware.org/index.php?title=Talk:CH391L/S13/QuorumSensing&diff=681940&oldid=prev Benjamin Gilman at 04:29, 8 March 2013 2013-03-08T04:29:17Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 04:29, 8 March 2013</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 5:</td> <td colspan="2" class="diff-lineno">Line 5:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**'''[[User:Neil R Gottel|Neil R Gottel]] 19:29, 7 March 2013 (EST)''':With regards to your second question, removing the bacterium's biofilm abilities would certainly reduce it's ability to survive within the body. This is sorta unrelated, but I just came across this paper showing how to [http://www.pnas.org/content/104/27/11197.full use phage to break apart biofilms]. Simply have the phage carry a gene for an enzyme that targets the main structural support of the film, so that infected cells produce large quantities of it. When the cell lyses, the enzymes are released, and expose more bacteria to the phage. Maybe my next topic for the class should be on the role of phage in synthetic biology...</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**'''[[User:Neil R Gottel|Neil R Gottel]] 19:29, 7 March 2013 (EST)''':With regards to your second question, removing the bacterium's biofilm abilities would certainly reduce it's ability to survive within the body. This is sorta unrelated, but I just came across this paper showing how to [http://www.pnas.org/content/104/27/11197.full use phage to break apart biofilms]. Simply have the phage carry a gene for an enzyme that targets the main structural support of the film, so that infected cells produce large quantities of it. When the cell lyses, the enzymes are released, and expose more bacteria to the phage. Maybe my next topic for the class should be on the role of phage in synthetic biology...</div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">***'''[[User:Benjamin Gilman|Benjamin Gilman]] 23:29, 7 March 2013 (EST)''': I'll second the phage idea...</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''[[User:Aurko Dasgupta|Aurko Dasgupta]] 19:17, 7 March 2013 (EST)''': Isn't H<sub>2</sub>O<sub>2</sub> toxic to all sorts of creatures? How does the host system cope with a toxic communication method, or are concentrations used just too low? Does it have a fast catalase response in case it gets swamped with signal?</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''[[User:Aurko Dasgupta|Aurko Dasgupta]] 19:17, 7 March 2013 (EST)''': Isn't H<sub>2</sub>O<sub>2</sub> toxic to all sorts of creatures? How does the host system cope with a toxic communication method, or are concentrations used just too low? Does it have a fast catalase response in case it gets swamped with signal?</div></td></tr>  </table>

Benjamin Gilman http://openwetware.org/index.php?title=Talk:CH391L/S13/QuorumSensing&diff=681939&oldid=prev Benjamin Gilman at 04:28, 8 March 2013 2013-03-08T04:28:17Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 04:28, 8 March 2013</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 2:</td> <td colspan="2" class="diff-lineno">Line 2:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''[[User:Catherine I. Mortensen|Catherine I. Mortensen]] 11:24, 7 March 2013 (EST)''':Is it practical to use P. aeruginosa bacterium as a means of ridding the ocean of tarballs and oil considering this bacterium is lethal to organisms? If it was possible to rid P. aeruginosa's ability to create biofilm make the bacterium considerably less lethal?</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''[[User:Catherine I. Mortensen|Catherine I. Mortensen]] 11:24, 7 March 2013 (EST)''':Is it practical to use P. aeruginosa bacterium as a means of ridding the ocean of tarballs and oil considering this bacterium is lethal to organisms? If it was possible to rid P. aeruginosa's ability to create biofilm make the bacterium considerably less lethal?</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**'''[[User:Neil R Gottel|Neil R Gottel]] 19:29, 7 March 2013 (EST)''':You're refering to those wild strains of P. aeruginosa that were isolated from tarballs, right? I think P. aeruginosa is dangerous only if you have a compromised immune system, or something like cystic fibrosis. I suspect the P. aeurginosa that are living in tarballs probably aren't the same kind that would be infecting humans. The thing with oil spills is that they're naturally occurring (at very low levels, compared to something like Deepwater Horizon), so there's bacteria in the wild that derive their energy at least partially from hydrocarbons.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**'''[[User:Neil R Gottel|Neil R Gottel]] 19:29, 7 March 2013 (EST)''':You're refering to those wild strains of P. aeruginosa that were isolated from tarballs, right? I think P. aeruginosa is dangerous only if you have a compromised immune system, or something like cystic fibrosis. I suspect the P. aeurginosa that are living in tarballs probably aren't the same kind that would be infecting humans. The thing with oil spills is that they're naturally occurring (at very low levels, compared to something like Deepwater Horizon), so there's bacteria in the wild that derive their energy at least partially from hydrocarbons.</div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">***'''[[User:Benjamin Gilman|Benjamin Gilman]] 23:28, 7 March 2013 (EST)''': Pseudomonas aeruginosa is found pretty much everywhere thanks to the fact that it likes a huge range of surfaces and can grow in hypoxic conditions, so there are a ton of strains out there.  It's a health problem not because it's so infectious, but because you're constantly exposed to it anyway and it's hard to get rid of with antibiotics.  Even if the same strains were used for oil cleanup, I doubt you'd see more cases of it popping up.</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**'''[[User:Neil R Gottel|Neil R Gottel]] 19:29, 7 March 2013 (EST)''':With regards to your second question, removing the bacterium's biofilm abilities would certainly reduce it's ability to survive within the body. This is sorta unrelated, but I just came across this paper showing how to [http://www.pnas.org/content/104/27/11197.full use phage to break apart biofilms]. Simply have the phage carry a gene for an enzyme that targets the main structural support of the film, so that infected cells produce large quantities of it. When the cell lyses, the enzymes are released, and expose more bacteria to the phage. Maybe my next topic for the class should be on the role of phage in synthetic biology...</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>**'''[[User:Neil R Gottel|Neil R Gottel]] 19:29, 7 March 2013 (EST)''':With regards to your second question, removing the bacterium's biofilm abilities would certainly reduce it's ability to survive within the body. This is sorta unrelated, but I just came across this paper showing how to [http://www.pnas.org/content/104/27/11197.full use phage to break apart biofilms]. Simply have the phage carry a gene for an enzyme that targets the main structural support of the film, so that infected cells produce large quantities of it. When the cell lyses, the enzymes are released, and expose more bacteria to the phage. Maybe my next topic for the class should be on the role of phage in synthetic biology...</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''[[User:Aurko Dasgupta|Aurko Dasgupta]] 19:17, 7 March 2013 (EST)''': Isn't H<sub>2</sub>O<sub>2</sub> toxic to all sorts of creatures? How does the host system cope with a toxic communication method, or are concentrations used just too low? Does it have a fast catalase response in case it gets swamped with signal?</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''[[User:Aurko Dasgupta|Aurko Dasgupta]] 19:17, 7 March 2013 (EST)''': Isn't H<sub>2</sub>O<sub>2</sub> toxic to all sorts of creatures? How does the host system cope with a toxic communication method, or are concentrations used just too low? Does it have a fast catalase response in case it gets swamped with signal?</div></td></tr>  </table>

Benjamin Gilman http://openwetware.org/index.php?title=Talk:CH391L/S13/QuorumSensing&diff=681862&oldid=prev Neil R Gottel at 00:29, 8 March 2013 2013-03-08T00:29:58Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 00:29, 8 March 2013</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''[[User:Kevin Baldridge|Kevin Baldridge]] 16:45, 6 March 2013 (EST)''':There are some formatting issues that should be addressed. For example, the arsenic sensing section has two figures that cannot fit side by side as is, so you should reduce the size of one or maybe fill in some of the blank space with text. The "multiple sensing" section looks like a good model for the others, the text and images fit together nicely.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''[[User:Kevin Baldridge|Kevin Baldridge]] 16:45, 6 March 2013 (EST)''':There are some formatting issues that should be addressed. For example, the arsenic sensing section has two figures that cannot fit side by side as is, so you should reduce the size of one or maybe fill in some of the blank space with text. The "multiple sensing" section looks like a good model for the others, the text and images fit together nicely.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''[[User:Catherine I. Mortensen|Catherine I. Mortensen]] 11:24, 7 March 2013 (EST)''':Is it practical to use P. aeruginosa bacterium as a means of ridding the ocean of tarballs and oil considering this bacterium is lethal to organisms? If it was possible to rid P. aeruginosa's ability to create biofilm make the bacterium considerably less lethal?</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''[[User:Catherine I. Mortensen|Catherine I. Mortensen]] 11:24, 7 March 2013 (EST)''':Is it practical to use P. aeruginosa bacterium as a means of ridding the ocean of tarballs and oil considering this bacterium is lethal to organisms? If it was possible to rid P. aeruginosa's ability to create biofilm make the bacterium considerably less lethal?</div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">**'''[[User:Neil R Gottel|Neil R Gottel]] 19:29, 7 March 2013 (EST)''':You're refering to those wild strains of P. aeruginosa that were isolated from tarballs, right? I think P. aeruginosa is dangerous only if you have a compromised immune system, or something like cystic fibrosis. I suspect the P. aeurginosa that are living in tarballs probably aren't the same kind that would be infecting humans. The thing with oil spills is that they're naturally occurring (at very low levels, compared to something like Deepwater Horizon), so there's bacteria in the wild that derive their energy at least partially from hydrocarbons.</ins></div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">**'''[[User:Neil R Gottel|Neil R Gottel]] 19:29, 7 March 2013 (EST)''':With regards to your second question, removing the bacterium's biofilm abilities would certainly reduce it's ability to survive within the body. This is sorta unrelated, but I just came across this paper showing how to [http://www.pnas.org/content/104/27/11197.full use phage to break apart biofilms]. Simply have the phage carry a gene for an enzyme that targets the main structural support of the film, so that infected cells produce large quantities of it. When the cell lyses, the enzymes are released, and expose more bacteria to the phage. Maybe my next topic for the class should be on the role of phage in synthetic biology...</ins></div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''[[User:Aurko Dasgupta|Aurko Dasgupta]] 19:17, 7 March 2013 (EST)''': Isn't H<sub>2</sub>O<sub>2</sub> toxic to all sorts of creatures? How does the host system cope with a toxic communication method, or are concentrations used just too low? Does it have a fast catalase response in case it gets swamped with signal?</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''[[User:Aurko Dasgupta|Aurko Dasgupta]] 19:17, 7 March 2013 (EST)''': Isn't H<sub>2</sub>O<sub>2</sub> toxic to all sorts of creatures? How does the host system cope with a toxic communication method, or are concentrations used just too low? Does it have a fast catalase response in case it gets swamped with signal?</div></td></tr>  </table>

Neil R Gottel http://openwetware.org/index.php?title=Talk:CH391L/S13/QuorumSensing&diff=681859&oldid=prev Aurko Dasgupta at 00:17, 8 March 2013 2013-03-08T00:17:53Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 00:17, 8 March 2013</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''[[User:Kevin Baldridge|Kevin Baldridge]] 16:45, 6 March 2013 (EST)''':There are some formatting issues that should be addressed. For example, the arsenic sensing section has two figures that cannot fit side by side as is, so you should reduce the size of one or maybe fill in some of the blank space with text. The "multiple sensing" section looks like a good model for the others, the text and images fit together nicely.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''[[User:Kevin Baldridge|Kevin Baldridge]] 16:45, 6 March 2013 (EST)''':There are some formatting issues that should be addressed. For example, the arsenic sensing section has two figures that cannot fit side by side as is, so you should reduce the size of one or maybe fill in some of the blank space with text. The "multiple sensing" section looks like a good model for the others, the text and images fit together nicely.</div></td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''[[User:Catherine I. Mortensen|Catherine I. Mortensen]] 11:24, 7 March 2013 (EST)''':Is it practical to use P. aeruginosa bacterium as a means of ridding the ocean of tarballs and oil considering this bacterium is lethal to organisms? If it was possible to rid P. aeruginosa's ability to create biofilm make the bacterium considerably less lethal?</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''[[User:Catherine I. Mortensen|Catherine I. Mortensen]] 11:24, 7 March 2013 (EST)''':Is it practical to use P. aeruginosa bacterium as a means of ridding the ocean of tarballs and oil considering this bacterium is lethal to organisms? If it was possible to rid P. aeruginosa's ability to create biofilm make the bacterium considerably less lethal?</div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*'''[[User:Aurko Dasgupta|Aurko Dasgupta]] 19:17, 7 March 2013 (EST)''': Isn't H<sub>2</sub>O<sub>2</sub> toxic to all sorts of creatures? How does the host system cope with a toxic communication method, or are concentrations used just too low? Does it have a fast catalase response in case it gets swamped with signal?</ins></div></td></tr>  </table>

Aurko Dasgupta http://openwetware.org/index.php?title=Talk:CH391L/S13/QuorumSensing&diff=681677&oldid=prev Catherine I. Mortensen at 16:24, 7 March 2013 2013-03-07T16:24:25Z

<p></p> <table style="background-color: white; color:black;"> <col class='diff-marker' /> <col class='diff-content' /> <col class='diff-marker' /> <col class='diff-content' /> <tr valign='top'> <td colspan='2' style="background-color: white; color:black;">←Older revision</td> <td colspan='2' style="background-color: white; color:black;">Revision as of 16:24, 7 March 2013</td> </tr> <tr><td colspan="2" class="diff-lineno">Line 1:</td> <td colspan="2" class="diff-lineno">Line 1:</td></tr> <tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''[[User:Kevin Baldridge|Kevin Baldridge]] 16:45, 6 March 2013 (EST)''':There are some formatting issues that should be addressed. For example, the arsenic sensing section has two figures that cannot fit side by side as is, so you should reduce the size of one or maybe fill in some of the blank space with text. The "multiple sensing" section looks like a good model for the others, the text and images fit together nicely.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>*'''[[User:Kevin Baldridge|Kevin Baldridge]] 16:45, 6 March 2013 (EST)''':There are some formatting issues that should be addressed. For example, the arsenic sensing section has two figures that cannot fit side by side as is, so you should reduce the size of one or maybe fill in some of the blank space with text. The "multiple sensing" section looks like a good model for the others, the text and images fit together nicely.</div></td></tr> <tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*'''[[User:Catherine I. Mortensen|Catherine I. Mortensen]] 11:24, 7 March 2013 (EST)''':Is it practical to use P. aeruginosa bacterium as a means of ridding the ocean of tarballs and oil considering this bacterium is lethal to organisms? If it was possible to rid P. aeruginosa's ability to create biofilm make the bacterium considerably less lethal?</ins></div></td></tr>  </table>

Catherine I. Mortensen