Talk:CH391L/S13/Metabolic Engineering and Thermophiles

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*'''[[User:Evan J. Weaver|Evan Weaver]] 13:04, 16 April 2013 (EDT)''':  What makes thermophiles so hard to culture?  Is it because of the high heat in combination with the amount of pressure needed to keep them alive?
*'''[[User:Evan J. Weaver|Evan Weaver]] 13:04, 16 April 2013 (EDT)''':  What makes thermophiles so hard to culture?  Is it because of the high heat in combination with the amount of pressure needed to keep them alive?
**'''[[User:Aurko Dasgupta|Aurko Dasgupta]] 18:44, 16 April 2013 (EDT)''':I think thermophiles typically have a slower growth rate that mesotherms like ''E. coli''. ''[http://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans]'' (not a thermophile, but another kind of extremophile) takes 2-3 days to form decent sized colonies on plates. I think the difficulty of transformation is also partially due to differences in their exponential phase compared to normal bacteria.
**'''[[User:Aurko Dasgupta|Aurko Dasgupta]] 18:44, 16 April 2013 (EDT)''':I think thermophiles typically have a slower growth rate that mesotherms like ''E. coli''. ''[http://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans]'' (not a thermophile, but another kind of extremophile) takes 2-3 days to form decent sized colonies on plates. I think the difficulty of transformation is also partially due to differences in their exponential phase compared to normal bacteria.
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*'''[[User:Gabriel Wu|Gabriel Wu]] 23:07, 17 April 2013 (EDT)''': What's the cost-benefit of maintaing a higher culturing temperature versus using other forms of selection? I know antibiotics at industrial scale are cost prohibitive. What selection is used currently and is it really cheaper to just keep cultures at 50°C?

Revision as of 23:07, 17 April 2013

  • Gabriel Wu 17:11, 15 April 2013 (EDT): The latest and greatest on the artemisinin project.
  • Dwight Tyler Fields 17:31, 15 April 2013 (EDT): From a big picture perspective, what are the boundaries on what one can get cells to produce? Maybe you could include this in a brief section on "future challenges" in the field?
  • Evan Weaver 13:04, 16 April 2013 (EDT): What makes thermophiles so hard to culture? Is it because of the high heat in combination with the amount of pressure needed to keep them alive?
    • Aurko Dasgupta 18:44, 16 April 2013 (EDT):I think thermophiles typically have a slower growth rate that mesotherms like E. coli. Deinococcus radiodurans (not a thermophile, but another kind of extremophile) takes 2-3 days to form decent sized colonies on plates. I think the difficulty of transformation is also partially due to differences in their exponential phase compared to normal bacteria.
  • Gabriel Wu 23:07, 17 April 2013 (EDT): What's the cost-benefit of maintaing a higher culturing temperature versus using other forms of selection? I know antibiotics at industrial scale are cost prohibitive. What selection is used currently and is it really cheaper to just keep cultures at 50°C?
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