Talk:CH391L/S13/Metabolic Engineering and Thermophiles

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 2: Line 2:
*'''[[User:Dwight Tyler Fields|Dwight Tyler Fields]] 17:31, 15 April 2013 (EDT)''': From a big picture perspective, what are the boundaries on what one can get cells to produce? Maybe you could include this in a brief section on "future challenges" in the field?
*'''[[User:Dwight Tyler Fields|Dwight Tyler Fields]] 17:31, 15 April 2013 (EDT)''': From a big picture perspective, what are the boundaries on what one can get cells to produce? Maybe you could include this in a brief section on "future challenges" in the field?
*'''[[User:Evan J. Weaver|Evan Weaver]] 13:04, 16 April 2013 (EDT)''':  What makes thermophiles so hard to culture?  Is it because of the high heat in combination with the amount of pressure needed to keep them alive?
*'''[[User:Evan J. Weaver|Evan Weaver]] 13:04, 16 April 2013 (EDT)''':  What makes thermophiles so hard to culture?  Is it because of the high heat in combination with the amount of pressure needed to keep them alive?
-
**'''[[User:Aurko Dasgupta|Aurko Dasgupta]] 18:44, 16 April 2013 (EDT)''':I think thermophiles typically have a slower growth rate that mesotherms like ''E. coli''. ''Deinococcus radiodurans'' (not a thermophile, but another kind of extremophile) takes 2-3 days to form decent sized colonies on plates. I think the difficulty of transformation is also partially due to differences in their exponential phase compared to normal bacteria.
+
**'''[[User:Aurko Dasgupta|Aurko Dasgupta]] 18:44, 16 April 2013 (EDT)''':I think thermophiles typically have a slower growth rate that mesotherms like ''E. coli''. ''[http://en.wikipedia.org/wiki/Deinococcus_radiodurans Deinococcus radiodurans]'' (not a thermophile, but another kind of extremophile) takes 2-3 days to form decent sized colonies on plates. I think the difficulty of transformation is also partially due to differences in their exponential phase compared to normal bacteria.

Revision as of 17:45, 16 April 2013

  • Gabriel Wu 17:11, 15 April 2013 (EDT): The latest and greatest on the artemisinin project.
  • Dwight Tyler Fields 17:31, 15 April 2013 (EDT): From a big picture perspective, what are the boundaries on what one can get cells to produce? Maybe you could include this in a brief section on "future challenges" in the field?
  • Evan Weaver 13:04, 16 April 2013 (EDT): What makes thermophiles so hard to culture? Is it because of the high heat in combination with the amount of pressure needed to keep them alive?
    • Aurko Dasgupta 18:44, 16 April 2013 (EDT):I think thermophiles typically have a slower growth rate that mesotherms like E. coli. Deinococcus radiodurans (not a thermophile, but another kind of extremophile) takes 2-3 days to form decent sized colonies on plates. I think the difficulty of transformation is also partially due to differences in their exponential phase compared to normal bacteria.
Personal tools