Talk:CH391L/S13/In vitro Selection of FNAs

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FNAs Talk

  • Kevin Baldridge 17:24, 11 February 2013 (EST):The first thing I see is that small non-coding RNAs are not represented in this group. Maybe it would be a good idea to add a section about prokaryotic small RNAs or eukaryotic micro- or piwi-interacting RNAs that are involved in pre-translational regulation.
    • Alvaro E. Rodriguez M. 21:16, 11 February 2013 (EST):Hello Kevin he reason why none of the small non-coding RNAs are mentioned is that they do no perform any type of catalysis/ligand binding on their own.
  • Benjamin Gilman 17:59, 11 February 2013 (EST): There's been been some work done on evolving deoxyribozymes (with or without unnatural bases) that catalyze a range of organic and inorganic synthesis reactions. Checking out some papers from Bruce Eaton might be a good place to start.
    • Alvaro E. Rodriguez M. 21:16, 11 February 2013 (EST): Hey, Ben do you happen to have a copy of the RNA world article were you mention RNase P as being discovered much earlier. I also appreciate the link to the Bruce Eaton page as it would be helpful to include his work in this wiki page.
      • Benjamin Gilman 12:30, 14 February 2013 (EST): I said that I thought RNase P was discovered before the tetrahymena group I intron, but I think it took a little while to figure out that the RNA portion of it was responsible for catalysis. The best place to look wouldn't be the RNA world, it would be Sidney Altman's original papers about it, or any of the many reviews he's written since. I'd bet that Jeff owns a copy of the RNA world though if you want to check it out. The life science library also has it.
  • Gabriel Wu 02:10, 14 February 2013 (EST): You have a note to self about including a discussion on RNA world. Include or move to talk section.
  • Gabriel Wu 02:10, 14 February 2013 (EST): Ribozyme section is numbered twice.
  • Gabriel Wu 02:10, 14 February 2013 (EST): Could you add a conclusion and/or a future directions section to put the topic in context?
  • Max E. Rubinson 11:03, 14 February 2013 (EST): Can you update this page to include a more detailed discussion of in vitro selection methods and maybe discuss some potential applications of functional nucleic acids?
  • User:Evan J WeaverIEvan Weaver 17:52, 14 February 2013 (CST): Could you put more links for ease of reading? I find that if makes life much easier when there are good sources in a wiki page. Also, could you better explain the different ribozymes in the classification? A useful link or short explanation would be nice. I also added a comma.
  • Aurko Dasgupta 21:38, 14 February 2013 (EST):Is the 2'OH the key to RNA's greater catalytic tendencies? Does it play a role in RNA's significantly lower stability compared to DNA?
    • User:Evan J WeaverIEvan Weaver 21:52, 15 February 2013 (CST): The 2' hydroxyl group can react with the phosphate group in the RNA backbone to cleave the RNA. Not sure about RNA catalysis though.
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