Talk:CH391L/S12/Protein regulators of transcription: Difference between revisions
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*'''[[User:Brian Renda|Brian Renda]] 17:23, 5 March 2012 (EST)''':to add: gal, tet on, off, copper, m-toluic acid | *'''[[User:Brian Renda|Brian Renda]] 17:23, 5 March 2012 (EST)''':to add: gal, tet on, off, copper, m-toluic acid | ||
*'''[[User:Jeffrey E. Barrick|Jeffrey E. Barrick]] 12:18, 7 March 2012 (EST)''':There is one distinction between Lac and Ara regulation that is important. Then the regulatory protein is absent in the Lac system (for example, missing from a strain), your promoter is constitutively ON. Then the regulatory protein from the Ara system is absent, your promoter is constitutively OFF. These states will also result if a mutation happens that knocks out the regulatory protein, which can make the systems differentially robust to mutations. |
Revision as of 10:18, 7 March 2012
- Brian Renda 17:23, 5 March 2012 (EST):to add: gal, tet on, off, copper, m-toluic acid
- Jeffrey E. Barrick 12:18, 7 March 2012 (EST):There is one distinction between Lac and Ara regulation that is important. Then the regulatory protein is absent in the Lac system (for example, missing from a strain), your promoter is constitutively ON. Then the regulatory protein from the Ara system is absent, your promoter is constitutively OFF. These states will also result if a mutation happens that knocks out the regulatory protein, which can make the systems differentially robust to mutations.