Talk:CH391L/S12/MetabolicEngineering: Difference between revisions
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One interesting strategy for optimizing flux through a pathway is the design of protein scaffolds which spatially recruit components of a metabolic pathway. This "increases the effective concentration of each component of a pathway of interest", a useful property to increase flux without necessarily increasing expression of each component. Gabe Wu, a grad student in my year, is second author on this one. <cite> | One interesting strategy for optimizing flux through a pathway is the design of protein scaffolds which spatially recruit components of a metabolic pathway. This "increases the effective concentration of each component of a pathway of interest", a useful property to increase flux without necessarily increasing expression of each component. Gabe Wu, a grad student in my year, is second author on this one. <cite>WuPaper</cite> *'''[[User:Michael Hammerling|Michael Hammerling]] 11:26, 13 February 2012 (EST)''': | ||
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*'''[[User:Yi Kou|Yi Kou]] 02:17, 14 February 2012 (EST)''': I am just curious, suppose you get all necessary components for a kind of fuel, how do you adjust the concentration? or how do you extract one solvent from another (consider propanol from isobutanol, or hexane from ethyl acetate for esp)? Evaporation (even use rotavapor at fixed temp) would result in a mixture of some extent. | *'''[[User:Yi Kou|Yi Kou]] 02:17, 14 February 2012 (EST)''': I am just curious, suppose you get all necessary components for a kind of fuel, how do you adjust the concentration? or how do you extract one solvent from another (consider propanol from isobutanol, or hexane from ethyl acetate for esp)? Evaporation (even use rotavapor at fixed temp) would result in a mixture of some extent. | ||
==References== | |||
<biblio> | |||
#WuPaper pmid=19648908 | |||
</biblio> | |||
Revision as of 20:32, 18 February 2012
One interesting strategy for optimizing flux through a pathway is the design of protein scaffolds which spatially recruit components of a metabolic pathway. This "increases the effective concentration of each component of a pathway of interest", a useful property to increase flux without necessarily increasing expression of each component. Gabe Wu, a grad student in my year, is second author on this one. [1] *Michael Hammerling 11:26, 13 February 2012 (EST):
Things that would be good to address: 1) toxicity of intermediates and products in metabolic engineering, their effect on the host organisms, and the ways that this is dealt with. 2) What is the efficiency of the production of biofuels? Mass in to mass out? 3) what are the different feed stocks one uses? pros and cons of each?*Brian Renda 17:03, 13 February 2012 (EST):
- Yi Kou 02:17, 14 February 2012 (EST):I think considering the yield, it should be mole in&out.
- Jeffrey E. Barrick 22:30, 18 February 2012 (EST):That's the theoretical maximum yield, but it's difficult to ever achieve that, especially in these organic systems where you are sometimes trying to divert biomass production (that is, more cells) into yield of your bioproduct.
- Ben Slater 23:33, 13 February 2012 (EST): The University of Washington, last year's iGEM winner, had a project that seems to fit in with biofuels/metabolic engineering (results). I'd also recommend checking out the other half of their project, the gluten destruction component, just because it's cool.
- Peter Otoupal 14:36, 17 February 2012 (EST):The University of Nevada had a similar project if we're looking at teams who have worked on producing biofuels in the past. Yay biofuels!
- Jeffrey E. Barrick 22:30, 18 February 2012 (EST):That's a neat co-culture system.
- Yi Kou 02:17, 14 February 2012 (EST): I am just curious, suppose you get all necessary components for a kind of fuel, how do you adjust the concentration? or how do you extract one solvent from another (consider propanol from isobutanol, or hexane from ethyl acetate for esp)? Evaporation (even use rotavapor at fixed temp) would result in a mixture of some extent.
