Talk:CH391L/S12/Fluorescent Proteins: Difference between revisions
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*'''[[User:Jeffrey E. Barrick|Jeffrey E. Barrick]] 13:15, 25 March 2012 (EDT)''': Is there a destabilized GFP that works in bacteria in the iGEM registry? How does it work? | *'''[[User:Jeffrey E. Barrick|Jeffrey E. Barrick]] 13:15, 25 March 2012 (EDT)''': Is there a destabilized GFP that works in bacteria in the iGEM registry? How does it work? | ||
*'''[[User:Jeffrey E. Barrick|Jeffrey E. Barrick]] 13:20, 25 March 2012 (EDT)''': This statement under EGFP is confusing "the altered enzymes in EGFP". I believe they are talking about the expression of an enzyme that has been fused to EGFP in this case, since a common application of GFP is to fuse it to a protein to examine localization of that protein in the cell. |
Revision as of 10:20, 25 March 2012
- Joe Hanson 16:50, 19 March 2012 (EDT): Can you add something about Split GFP? It's a pretty cool system for things like solubility evolution, and it allows you to use smaller fusion proteins when compared to attaching the entire GFP ORF.
- Jeffrey E. Barrick 13:15, 25 March 2012 (EDT): Is there a destabilized GFP that works in bacteria in the iGEM registry? How does it work?
- Jeffrey E. Barrick 13:20, 25 March 2012 (EDT): This statement under EGFP is confusing "the altered enzymes in EGFP". I believe they are talking about the expression of an enzyme that has been fused to EGFP in this case, since a common application of GFP is to fuse it to a protein to examine localization of that protein in the cell.