Talk:BE.109:Protein engineering/Purifying beta-galactosidase: Difference between revisions
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| | | .7509x (fit to cross @ 0) R^2 = .9706 | ||
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Revision as of 08:52, 23 March 2006
To compare the purification protocols you have tried today, each team in each lab section should enter their data into the following tables.
Tuesday/Thursday
Team Color | purification method | equation for BSA standard curve | concentration of beta-gal stock | concentration of purified beta-gal (wild type) | activity of purified beta-gal (wild type) | concentration of modified beta-gal (list modification below table) | activity of modified beta-gal |
---|---|---|---|---|---|---|---|
Green | Ni-agarose | y=.7133x - .0244 | 2.08 mg/ml | .29 mg/ml | .158 mg/ml | ||
Purple | Paramagnetic Beads | OD-595 = 0.5556(Conc.) - 0.0012 | 4.19 mg/mL | 0.0501 mg/mL | 37.37 units | 0.0506 mg/mL | 34.87 units |
Red | N-agarose | .7509x (fit to cross @ 0) R^2 = .9706 | |||||
Blue! | Ni-agarose | 0.6709x-0.0002 | 3.75mg/mL | 0.647 | 0.0318 | 0.322 | 0.233 |
Pink | Ni-agarose | y=1.2x+0.0002 | 2.332 mg/mL | 0.111 mg/mL | OD 420= 0.7363 , Units = 2.6783 | 0.268 mg/mL | OD 420= 0.2173, Units= 1.8339 |
Yellow | paramagnetic beads | y = 1.11x-.06 | .55 mg/mL | .50 mg/mL | OD420=.56, Units = 48890.86 | .61 mg/mL | OD420=.63, Units = 34878.04 |
Wednesday/Friday
Team Color | purification method | equation for BSA standard curve | concentration of beta-gal stock | concentration of purified beta-gal (wild type) | activity of purified beta-gal (wild type) | concentration of modified beta-gal (list modification below table) | activity of modified beta-gal |
---|---|---|---|---|---|---|---|
Green | |||||||
Purple | |||||||
Red | |||||||
Blue | |||||||
Pink | |||||||
Yellow |