team color
|
candidate number
|
sequence
|
relative strength
|
comments/notes
|
Green
|
A
|
XXWAPAXXXXXX
|
1/18 of pCT-CON
|
This sequence did not show significant binding ability, although because of the poor sequencing data, we can not make any definite conclusions on the cause of the poor gold binding ability.
|
Green
|
B
|
XXXXVGXXXXXX
|
3.5 of pCT-CON
|
This sequence has some gold binding ability, however unlike D, the sequence data obtained for this colony is quite poor. As a result, we can not make any definite conclusions on what lead to it's binding.
|
Green
|
C
|
XXXXXXXXSLFL
|
.83 of pCT-CON
|
This sequence did not show significant binding ability, although because of the poor sequencing data, we can not make any definite conclusions on the cause of the poor gold binding ability.
|
Green
|
D
|
LRXXTXGLSLCR
|
7.83 of pCT-CON
|
Colony D was the only one from which we got some sort of meaningful sequence data from. Luckily, it was also our strongest binder. The sequence data shows a prevalence of Yellow Aliphatic Groups (Leucine) and Green Hydroxyl Groups (Serine), which may be important for gold binding.
|
Purple
|
B
|
WRxxxACRCVxx
|
0.3 x pCT-CON
|
2 cysteines may be signficant
|
Purple
|
C
|
MYYVAASxSVLM
|
2 x pCT-CON
|
2 methionines and 2 serines may be significant
|
Purple
|
D
|
*LARPVA*xxIx
|
10 x pCT-CON
|
best binder. stop codons are TAG and TGA
|
Yellow
|
A
|
EXXXPIXXXXXX
|
1.89 of pCT-CON
|
We do not know very much about sequence A, but we hypothesize that it contains aromatic amino acids. Here is a color coded chart of our sequences:
|
Yellow
|
B
|
RXRLRVXXXXVR
|
0.413 of pCT-CON
|
This sequence is the weakest gold binder and also exhibits the most amount of basic amino acids, which indicates that basic amino acids may prevent gold binding. Sequence A shows no basic amino acids, and sequence D contains two basic amino acids which may be the reason that sequence A bonds gold more strongly than sequence D, despite its lack of aromatic amino acids.
|
Yellow
|
C
|
EGXXPXXXXXXX
|
0.55 of pCT-CON
|
We do not know very much about sequence C. One observation that we had about sequences B and C is that they contained no aromatic residues, but they did contain acidic amino acids. But there is still even a chance that C and A are identical.
|
Yellow
|
D
|
FWCQSRWXEILV
|
1.72 of pCT-CON
|
This sequence contains three aromatic residues, which is rare in the library, which is a significant feature. Also, the weakly binding sequences (B and C) do not show any aromatic residues, which indicates that aromatic residues may be responsible for gold binding.
|
Blue!
|
A
|
LCVIQAXXKSIV
|
relatively good
|
Binding: more colonies than pCT-CON, but much less than on pAu1. Sequence: This sequence contains lots of aliphatic residues, and one cysteine.
|
Blue!
|
B
|
XXXXXPXXXRXX
|
not good
|
Binding: As many colonies formed as pCT-CON. Sequence: We do not have enough sequence data to comment on this sequence.
|
Blue!
|
C
|
XXXXXXXXXXAX
|
good binder
|
Binding: More colonies than pCT-CON, but much less than on pAu1. Sequence: We do not have enough sequence data to comment on this sequence.
|
Blue!
|
D
|
LXXXXXXXGMGX
|
good binder
|
Binding: More colonies than pCT-CON, but much less than on pAu1. Sequence: We do not have enough sequence data to comment on this sequence (has at least two Glycines though!).
|
Pink
|
A
|
VAASRLVCCXXXL
|
6 x pCT-CON
|
2 cysteines right next to each other might mean that disulfide bonds cannot occur so sulfur available to bind to gold.
|
Pink
|
D
|
XXADKLLXHAPQ*
|
6 x pCT-CON
|
Histidine present which if we enrinched the species, histidine would probably be present in greater amounts :)
|
Red
|
A
|
PDGT*LXVXXXX
|
3.125 x pCT-CON
|
Very similar to Sequence D. Similar relative strength to D. Has a proline next to a aspartic acid (acidic AA).
|
Red
|
B
|
DXXHPEYGPHCK
|
14.375 x pCT-CON
|
Very similar to sequence C. Similar relative strength to C. Has proline next to glutamic acid (acidic AA). Also has a proline further down sequence.
|
Red
|
C
|
XXXHPEYGPPXK
|
9.5 x pCT-CON
|
Very similar to sequence B. Similar relative strength to B. Has proline next to glutamic acid, and 2 prolines further down sequence next to each other.
|
Red
|
D
|
PDGT*LYXXXLX
|
.5 x pCT-CON
|
Very similar to sequence A. Similar relative strength to A. Has Proline next to aspartic acid (AA). --- Commonality between all four library sequences is presence of proline next to acidic AA, as well as extra prolines further into sequence.
|