Talk:Addition of 3' A overhangs to PCR products

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Revision as of 22:05, 2 May 2012 by Gwen (talk | contribs) (New page: There's no need to purify the product after the PCR step if you want to directly clone into the TA vector. Invitrogen's protocol calls for addition of .5U of Taq for a 10 minute incubation...)
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There's no need to purify the product after the PCR step if you want to directly clone into the TA vector. Invitrogen's protocol calls for addition of .5U of Taq for a 10 minute incubation @ 72C after the PCR is complete. It calls for a phenol extraction afterwards, but the product can be directly used to go into a TA vector ligation.

If the PCR product has already been purified, then this is an excellent protocol to follow.

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