Talk:20.109(S14):Complete Western and prepare damaged DNA (Day4): Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
Line 4: Line 4:


===W/F===
===W/F===
Recall that lane �10 is singly digested (with ''EcoRV'') DNA prepared by Su for a size comparison to the full-length plasmid versus plasmid with no insert.
[[Image:M2D4-Gel3_Grn-Ylw-Org-Su | right |thumb|250px | W/F Gel 3]]
[[Image:M2D4-Gel1_Pnk-Red-Blu-Su | left |thumb|250px | W/F Gel 1]]
[[Image:M2D4-Gel2_Wht-Sil-Prp-Su | center |thumb|250px | W/F Gel 2]]
[[Image:M2D4-Gel2.2_Wht | right |thumb|250px | W/F Gel 2 extra run time]]


==Westerns==
==Westerns==

Revision as of 11:16, 26 March 2014

DNA gels

T/R

W/F

Recall that lane �10 is singly digested (with EcoRV) DNA prepared by Su for a size comparison to the full-length plasmid versus plasmid with no insert.

File:M2D4-Gel3 Grn-Ylw-Org-Su
W/F Gel 3
File:M2D4-Gel1 Pnk-Red-Blu-Su
W/F Gel 1
File:M2D4-Gel2 Wht-Sil-Prp-Su
W/F Gel 2
File:M2D4-Gel2.2 Wht
W/F Gel 2 extra run time

Westerns

T/R

W/F

Hi folks -- I think these got flipped because they were sent from my phone. Please turn them in the usual orientation for use in your reports.

W/F Gel, Yellow group
W/F Gel, Red group
W/F Gel, Orange group
W/F Gel, Pink group
W/F Gel, Green group
W/F Gel, Blue group
W/F Gel, White group
W/F Gel, Purple group
W/F Gel, Silver group