Talk:20.109(S14):Complete Western and prepare damaged DNA (Day4)

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(W/F)
Current revision (10:27, 2 April 2014) (view source)
(DNA gels)
 
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===T/R===
===T/R===
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[[Image:TR_M2D4_gel1_S14.jpg | left |thumb|275px|T/R Gel 1]]
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[[Image:M2D4-Gel2_Pnk-Grn-Blu.jpg | right |thumb|275px | T/R Gel 2]]
 +
<br style="clear:both;"/>
===W/F===
===W/F===
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 +
Recall that lane 10 is singly digested (with ''EcoRV'') DNA prepared by Su for a size comparison to the full-length plasmid versus plasmid with no insert.
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 +
[[Image:M2D4-Gel1_Pnk-Red-Blu-Su.jpg | left |thumb|325px | W/F Gel 1]]
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[[Image:M2D4-Gel2_Wht-Sil-Prp-Su.jpg | center |thumb|325px | W/F Gel 2]]
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[[Image:M2D4-Gel3_Grn-Ylw-Org-Su.jpg | left |thumb|325px | W/F Gel 3]]
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[[Image:M2D4-Gel2.2_Wht.jpg | center |thumb|325px | W/F Gel 2 extra run time]]
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<br style="clear:both;"/>
==Westerns==
==Westerns==
===T/R===
===T/R===
 +
 +
[[Image:S14_M2D4_TR-Grn.JPG | left |thumb|250px | T/R WB, Green group]]
 +
[[Image:S14_M2D4_TR-Blu.JPG | right |thumb|250px | T/R WB, Blue group]]
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[[Image:S14_M2D4_TR-Ylw.JPG | center |thumb|250px | T/R WB, Yellow group]]
 +
[[Image:S14_M2D4_TR-pink.JPG | right |thumb|250px | T/R WB, pink group]]
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[[Image:S14_M2D4_TR-red.JPG | right |thumb|250px | T/R WB, red group]]
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[[Image:S14_M2D4_TR-orange.JPG | right |thumb|250px | T/R WB, orange group]]
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<br style="clear:both;"/>
===W/F===
===W/F===
-
[[Image:S14_M2D4_WF_Ylw.JPG | right |thumb|250px | W/F Gel, Yellow group]]
+
Hi folks -- I think these got flipped because they were sent from my phone. Please turn them in the usual orientation (and crop) for use in your reports.
-
[[Image:S14_M2D4_WF_Red.JPG | left |thumb|250px | W/F Gel, Red group]]
+
 
-
[[Image:S14_M2D4_WF_Org.JPG | center |thumb|250px | W/F Gel, Orange group]]
+
[[Image:S14_M2D4_WF-Ylw.JPG | right |thumb|250px | W/F Gel, Yellow group]]
-
[[Image:S14_M2D4_WF_Pnk.JPG | right |thumb|250px | W/F Gel, Pink group]]
+
[[Image:S14_M2D4_WF-Red.JPG | left |thumb|250px | W/F Gel, Red group]]
-
[[Image:S14_M2D4_WF_Grn.JPG | left |thumb|250px | W/F Gel, Green group]]
+
[[Image:S14_M2D4_WF-Org.JPG | center |thumb|250px | W/F Gel, Orange group]]
-
[[Image:S14_M2D4_WF_Blu.JPG | center |thumb|250px | W/F Gel, Blue group]]
+
[[Image:S14_M2D4_WF-Pnk.JPG | right |thumb|250px | W/F Gel, Pink group]]
-
[[Image:S14_M2D4_WF_Wht.JPG | right |thumb|250px | W/F Gel, White group]]
+
[[Image:S14_M2D4_WF-Grn.JPG | left |thumb|250px | W/F Gel, Green group]]
-
[[Image:S14_M2D4_WF_Prp.JPG | left |thumb|250px | W/F Gel, Purple group]]
+
[[Image:S14_M2D4_WF-Blu.JPG | center |thumb|250px | W/F Gel, Blue group]]
-
[[Image:S14_M2D4_WF_Sil.JPG | center |thumb|250px | W/F Gel, Silver group]]
+
[[Image:S14_M2D4_WF-Wht.JPG | right |thumb|250px | W/F Gel, White group]]
 +
[[Image:S14_M2D4_WF-Prp.JPG | left |thumb|250px | W/F Gel, Purple group]]
 +
[[Image:S14_M2D4_WF-Sil.JPG | center |thumb|250px | W/F Gel, Silver group]]
<br style="clear:both;"/>
<br style="clear:both;"/>

Current revision

Contents

DNA gels

T/R

T/R Gel 1
T/R Gel 1
T/R Gel 2
T/R Gel 2


W/F

Recall that lane 10 is singly digested (with EcoRV) DNA prepared by Su for a size comparison to the full-length plasmid versus plasmid with no insert.

W/F Gel 1
W/F Gel 1
W/F Gel 2
W/F Gel 2
W/F Gel 3
W/F Gel 3
W/F Gel 2 extra run time
W/F Gel 2 extra run time


Westerns

T/R

T/R WB, Green group
T/R WB, Green group
T/R WB, Blue group
T/R WB, Blue group
T/R WB, Yellow group
T/R WB, Yellow group
T/R WB, pink group
T/R WB, pink group
T/R WB, red group
T/R WB, red group
T/R WB, orange group
T/R WB, orange group


W/F

Hi folks -- I think these got flipped because they were sent from my phone. Please turn them in the usual orientation (and crop) for use in your reports.

W/F Gel, Yellow group
W/F Gel, Yellow group
W/F Gel, Red group
W/F Gel, Red group
W/F Gel, Orange group
W/F Gel, Orange group
W/F Gel, Pink group
W/F Gel, Pink group
W/F Gel, Green group
W/F Gel, Green group
W/F Gel, Blue group
W/F Gel, Blue group
W/F Gel, White group
W/F Gel, White group
W/F Gel, Purple group
W/F Gel, Purple group
W/F Gel, Silver group
W/F Gel, Silver group


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