Talk:20.109(S14):Complete Western and prepare damaged DNA (Day4)

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(W/F)
Current revision (09:27, 2 April 2014) (view source)
(DNA gels)
 
(19 intermediate revisions not shown.)
Line 2: Line 2:
===T/R===
===T/R===
-
 
+
[[Image:TR_M2D4_gel1_S14.jpg | left |thumb|275px|T/R Gel 1]]
 +
[[Image:M2D4-Gel2_Pnk-Grn-Blu.jpg | right |thumb|275px | T/R Gel 2]]
 +
<br style="clear:both;"/>
===W/F===
===W/F===
 +
 +
Recall that lane 10 is singly digested (with ''EcoRV'') DNA prepared by Su for a size comparison to the full-length plasmid versus plasmid with no insert.
 +
 +
[[Image:M2D4-Gel1_Pnk-Red-Blu-Su.jpg | left |thumb|325px | W/F Gel 1]]
 +
[[Image:M2D4-Gel2_Wht-Sil-Prp-Su.jpg | center |thumb|325px | W/F Gel 2]]
 +
[[Image:M2D4-Gel3_Grn-Ylw-Org-Su.jpg | left |thumb|325px | W/F Gel 3]]
 +
[[Image:M2D4-Gel2.2_Wht.jpg | center |thumb|325px | W/F Gel 2 extra run time]]
 +
<br style="clear:both;"/>
==Westerns==
==Westerns==
===T/R===
===T/R===
 +
 +
[[Image:S14_M2D4_TR-Grn.JPG | left |thumb|250px | T/R WB, Green group]]
 +
[[Image:S14_M2D4_TR-Blu.JPG | right |thumb|250px | T/R WB, Blue group]]
 +
[[Image:S14_M2D4_TR-Ylw.JPG | center |thumb|250px | T/R WB, Yellow group]]
 +
[[Image:S14_M2D4_TR-pink.JPG | right |thumb|250px | T/R WB, pink group]]
 +
[[Image:S14_M2D4_TR-red.JPG | right |thumb|250px | T/R WB, red group]]
 +
[[Image:S14_M2D4_TR-orange.JPG | right |thumb|250px | T/R WB, orange group]]
 +
<br style="clear:both;"/>
===W/F===
===W/F===
 +
 +
Hi folks -- I think these got flipped because they were sent from my phone. Please turn them in the usual orientation (and crop) for use in your reports.
[[Image:S14_M2D4_WF-Ylw.JPG | right |thumb|250px | W/F Gel, Yellow group]]
[[Image:S14_M2D4_WF-Ylw.JPG | right |thumb|250px | W/F Gel, Yellow group]]

Current revision

Contents

DNA gels

T/R

T/R Gel 1
T/R Gel 1
T/R Gel 2
T/R Gel 2


W/F

Recall that lane 10 is singly digested (with EcoRV) DNA prepared by Su for a size comparison to the full-length plasmid versus plasmid with no insert.

W/F Gel 1
W/F Gel 1
W/F Gel 2
W/F Gel 2
W/F Gel 3
W/F Gel 3
W/F Gel 2 extra run time
W/F Gel 2 extra run time


Westerns

T/R

T/R WB, Green group
T/R WB, Green group
T/R WB, Blue group
T/R WB, Blue group
T/R WB, Yellow group
T/R WB, Yellow group
T/R WB, pink group
T/R WB, pink group
T/R WB, red group
T/R WB, red group
T/R WB, orange group
T/R WB, orange group


W/F

Hi folks -- I think these got flipped because they were sent from my phone. Please turn them in the usual orientation (and crop) for use in your reports.

W/F Gel, Yellow group
W/F Gel, Yellow group
W/F Gel, Red group
W/F Gel, Red group
W/F Gel, Orange group
W/F Gel, Orange group
W/F Gel, Pink group
W/F Gel, Pink group
W/F Gel, Green group
W/F Gel, Green group
W/F Gel, Blue group
W/F Gel, Blue group
W/F Gel, White group
W/F Gel, White group
W/F Gel, Purple group
W/F Gel, Purple group
W/F Gel, Silver group
W/F Gel, Silver group


Personal tools