Talk:20.109(S14):Cell preparation for DNA repair assays (Day5)
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Concentrations of Student Prepped DNA
Note: All TA prepped DNA will be at 50 ng/μL.
| Cut topology | Group 1 | Concentration (ng/uL) | Group 2 | Concentration (ng/uL) | (Group 3) | (Concentration (ng/uL)) |
|---|---|---|---|---|---|---|
| Sticky ends, 5' overhangs | Orange Team (TR) | 27.0 | Red Team (WF) | 18.7 | ||
| Sticky ends, 3' overhangs | Blue Team (TR) | 28.5 | Purple Team (WF) | 11.0 | ||
| Blunt | Yellow Team (TR) | 21.4 | Yellow Team (WF) | 27.3 | Blue Team (WF) | 34.0 |
| Hybrid: 5' sticky upstream, blunt downstream | Pink Team (TR) | 34.9 | Green Team (WF) | 34.6 | ||
| Hybrid: 3' sticky upstream, blunt downstream | Red Team (TR) | 21.8 | Pink Team (WF) | 51.8 | ||
| Sticky ends with sequence mismatch | Green Team (TR) | 29.7 | White Team (WF) | 18.1 | ||
| Sticky ends with sequence and topology mismatch | BA Orange Team (WF) | 12.6 | not so BA Silver Team (WF) | 33.7 |
