Talk:20.109(S14):Cell preparation for DNA repair assays (Day5)
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Concentrations of TA Prepped DNA
| Cut topology | Group 1 | Concentration (ng/uL) | Group 2 | Concentration (ng/uL) | (Group 3) | (Concentration (ng/uL)) | |
|---|---|---|---|---|---|---|---|
| Sticky ends, 5' overhangs | Orange Team (TR) | 65.1 | Red Team (WF) | 59.5 | |||
| Sticky ends, 3' overhangs | Blue Team (TR) | 73.9 | Purple Team (WF) | 60.6 | |||
| Blunt | Yellow Team (TR) | 67.7 | Yellow Team (WF) | 60.7 | Blue Team (WF) | 60.4 | |
| Hybrid: 5' sticky upstream, blunt downstream | Pink Team (TR) | 65.9 | Green Team (WF) | 62.7 | |||
| Hybrid: 3' sticky upstream, blunt downstream | Red Team (TR) | 65.5 | Pink Team (WF) | 74.3 | |||
| Sticky ends with sequence mismatch | Green Team (TR) | 65.6 | White Team (WF) | 62.9 | |||
| Sticky ends with sequence and topology mismatch | BA Orange Team (WF) | 62.8 | not so BA Silver Team (WF) | 53.0 |
