Talk:20.109(S14):Cell preparation for DNA repair assays (Day5)

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Line 14: Line 14:
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| Red Team (WF)
| Red Team (WF)
 +
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Line 19: Line 20:
| Sticky ends, 3' overhangs  
| Sticky ends, 3' overhangs  
|Blue Team (TR)
|Blue Team (TR)
 +
|
|Purple Team (WF)
|Purple Team (WF)
 +
|
 +
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|
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|-
|-
| Blunt  
| Blunt  
|Yellow Team (TR)
|Yellow Team (TR)
 +
|
|Yellow Team (WF)
|Yellow Team (WF)
 +
|
|Blue Team (WF)
|Blue Team (WF)
 +
|
|-
|-
| Hybrid: 5' sticky upstream, blunt downstream  
| Hybrid: 5' sticky upstream, blunt downstream  
|Pink Team (TR)
|Pink Team (TR)
 +
|
|Green Team (WF)
|Green Team (WF)
 +
|
 +
|
|
|
|-
|-
| Hybrid: 3' sticky upstream, blunt downstream  
| Hybrid: 3' sticky upstream, blunt downstream  
|Red Team (TR)
|Red Team (TR)
 +
|
|Pink Team (WF)
|Pink Team (WF)
 +
|
 +
|
|
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|-
|-
| Sticky ends with sequence mismatch  
| Sticky ends with sequence mismatch  
|Green Team (TR)
|Green Team (TR)
 +
|
|White Team (WF)
|White Team (WF)
 +
|
 +
|
|
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|-
|-
| Sticky ends with sequence and topology mismatch
| Sticky ends with sequence and topology mismatch
|BA Orange Team (WF)
|BA Orange Team (WF)
 +
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|not so BA Silver Team (WF)
|not so BA Silver Team (WF)
 +
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 +
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Revision as of 13:21, 28 March 2014

Concentrations of TA Prepped DNA

Cut topology Group 1 Concentration Group 2 Concentration (Group 3) (Concentration)
Sticky ends, 5' overhangs Orange Team (TR) Red Team (WF)
Sticky ends, 3' overhangs Blue Team (TR) Purple Team (WF)
Blunt Yellow Team (TR) Yellow Team (WF) Blue Team (WF)
Hybrid: 5' sticky upstream, blunt downstream Pink Team (TR) Green Team (WF)
Hybrid: 3' sticky upstream, blunt downstream Red Team (TR) Pink Team (WF)
Sticky ends with sequence mismatch Green Team (TR) White Team (WF)
Sticky ends with sequence and topology mismatch BA Orange Team (WF) not so BA Silver Team (WF)
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