# Talk:20.109(S13):Transcript-level analysis (Day6)

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 Revision as of 14:22, 6 May 2013 (view source) (→qPCR data)← Previous diff Revision as of 14:23, 6 May 2013 (view source) (→qPCR section-wide curves)Next diff → Line 14: Line 14: ==qPCR section-wide curves== ==qPCR section-wide curves== + + ===T/R=== [[Image:S13_M3D5_TR_raw.jpg|thumb|400px|left|T/R section, Raw amplification curves]] [[Image:S13_M3D5_TR_raw.jpg|thumb|400px|left|T/R section, Raw amplification curves]] Line 23: Line 25:

+ ===W/F=== [[Image:S13_M3D5_WF_raw.jpg|thumb|400px|left|W/F section, Raw amplification curves]] [[Image:S13_M3D5_WF_raw.jpg|thumb|400px|left|W/F section, Raw amplification curves]]

## qPCR data

### T/R

The Excel file below contains all of the T/R data. Two different CP values are given. The first is calculated when all samples are thresholded at the same level. The second is calculated when a separate threshold is made for each primer set. (T/R Purple, because your samples ran separately, there were not enough of them to make a per RNA threshold reasonable.)

### W/F

The Excel file below contains all of the T/R data. Two different CP values are given. The first is calculated when all samples are thresholded at the same level. The second is calculated when a separate threshold is made for each primer set.

## qPCR section-wide curves

### T/R

T/R section, Raw amplification curves
T/R section, Log-scale amplification curves with noise threshold
T/R section, Melt curves for 18 samples
T/R section, Melt curves for CN II samples
T/R section, Melt curves for CN II samples

### W/F

W/F section, Raw amplification curves
W/F section, Log-scale amplification curves with noise threshold
W/F section, Melt curves for 18 samples
W/F section, Melt curves for CN II samples
W/F section, Melt curves for CN II samples

## Cross-group meetings

### T/R Scheme

Red 1/Yellow 1: Marisol/Dominique

Red 2/Blue 1: Nick/Justin

Orange 1/Platinum 2: Daphne/Katie

Platinum 1/Blue 2: Emily/Noa

Yellow 2/Green 2: Ebaa/Jesse

Green 1/Purple 1:Nikki/Sarah

Orange 2/Purple 2: Stas/Mitali