Talk:20.109(S13):Transcript-level analysis (Day6)

qPCR data

T/R

The text file below contains all of the T/R data. Two different CP values are given. The first is calculated when all samples are thresholded at the same level. The second is calculated when a separate threshold is made for each primer set. (T/R Purple, because your samples ran separately, there were not enough of them to make a per RNA threshold reasonable.)

W/F

The text file below contains all of the T/R data. Two different CP values are given. The first is calculated when all samples are thresholded at the same level. The second is calculated when a separate threshold is made for each primer set.

qPCR section-wide curves

T/R section, Raw amplification curves

T/R section, Log-scale amplification curves with noise threshold

T/R section, Melt curves for 18 samples

T/R section, Melt curves for CN II samples

T/R section, Melt curves for CN II samples

W/F section, Raw amplification curves

W/F section, Log-scale amplification curves with noise threshold

W/F section, Melt curves for 18 samples

W/F section, Melt curves for CN II samples

W/F section, Melt curves for CN II samples

Cross-group meetings

Scheme

Red 1/Yellow 1

Red 2/Blue 1

Orange 1/Pink 2

Orange 2/Blue 2

Yellow 2/Green 2

Green 1/Purple 2

Pink 1/Purple 1