Talk:20.109(S13):Induce protein and evaluate DNA (Day5)

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(WF O/N Cultures and Gels)
(WF O/N Cultures and Gels)
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Revision as of 15:31, 4 April 2013

Contents

Induced pellet example

Examples of induced (left) and control (right) cell pellets
Examples of induced (left) and control (right) cell pellets


TR O/N Cultures and Gels

Below you should list the OD600 values of the induced cultures (stock value), as well as your pellet observations. (Is the color dull or bright? More yellow or more green?) The teaching faculty will fill in this information for the cultures needing to be grown overnight.

Group OD WT OD D24H/E67K/T79P/M124S OD Chosen mutant Pellets yellow?
Example 0.95 1.2 (D24H) 0.82 (G20P) Y, bright ylw-grn
Red 0.22 0.51 (D24H) 0.55 grn, grn, ylw (+hint of grn)
Orange 0.36 0.36 (D24H) 0.38 grn, grn, ylw (+hint of grn)
Yellow 0.39 2.12 (T79P) 3.36 (K77E2--not the chosen mutant)
extra is 0.17
bright yellow; ylw (+hint of grn)
Green 0.34 1.59 (T79P) 1.16 (M71R) Bright Yellow
Blue 0.035 0.036 (M124S) 0.088 (M71S) Y, bright ylw
Purple 0.30 0.58 (M124S) 0.32 ylw-grn, ylw-grn, grn
Platinum 0.52 0.75 (M124S) 0.38 ylw-grn, ylw-grn, grn

Gels

T/R Red
T/R Red
T/R Orange
T/R Orange
T/R Yellow
T/R Yellow
T/R Green
T/R Green
T/R Blue
T/R Blue
T/R Purple
T/R Purple
T/R Platinum
T/R Platinum


WF O/N Cultures and Gels

Below you should list the OD600 values of the induced cultures (stock value), as well as your pellet observations. (Is the color dull or bright? More yellow or more green?) The teaching faculty will fill in this information for the cultures needing to be grown overnight.

Group OD WT OD D24H/E67K/T79P/M124S OD mutant option 1 OD mutant option 2 Pellets yellow?
Example 0.95 1.2 (D24H) 0.82 (G20P) N/A Y, bright ylw-grn
Red
Orange
Yellow
Green
Blue
Pink
Purple
Platinum

Gels

W/F Orange
W/F Orange
W/F Yellow
W/F Yellow
W/F Green
W/F Green
W/F Blue
W/F Blue
W/F Pink
W/F Pink
W/F Purple
W/F Purple
W/F Platinum
W/F Platinum


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