Talk:20.109(S13):Induce protein and evaluate DNA (Day5)

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(New page: ==Induced pellet example== Examples of induced (left) and control (right) cell pellets <br style="clear:both;"/> ==TR O/N Culture...)
(TR O/N Cultures and Gels)
Line 45: Line 45:
|
|
|
|
 +
|-
|Blue
|Blue
|
|

Revision as of 13:53, 28 March 2013

Contents

Induced pellet example

Examples of induced (left) and control (right) cell pellets
Examples of induced (left) and control (right) cell pellets


TR O/N Cultures and Gels

Below you should list the OD600 values of the induced cultures (stock value), as well as your pellet observations. (Is the color dull or bright? More yellow or more green?) The teaching faculty will fill in this information for the cultures needing to be grown overnight.

Group OD WT OD D24H/E67K/T79P/M124S OD Chosen mutant Pellets yellow?
Example 0.95 1.2 (D24H) 0.82 (G20P) Y, bright ylw-grn
Red
Orange
Yellow
Green
Blue
Pink
Purple
Platinum

Gels

T/R Red
T/R Red
T/R Yellow
T/R Yellow
T/R Green
T/R Green
T/R Blue
T/R Blue
T/R Purple
T/R Purple
T/R Platinum
T/R Platinum


WF O/N Cultures and Gels

Below you should list the OD600 values of the induced cultures (stock value), as well as your pellet observations. (Is the color dull or bright? More yellow or more green?) The teaching faculty will fill in this information for the cultures needing to be grown overnight.

Group OD WT OD D24H/E67K/T79P/M124S OD Chosen mutant Pellets yellow?
Example 0.95 1.2 (D24H) 0.82 (G20P) Y, bright ylw-grn
Red
Orange
Yellow
Green Blue
Pink
Purple
Platinum

Gels

W/F Orange
W/F Orange
W/F Yellow
W/F Yellow
W/F Green
W/F Green
W/F Blue
W/F Blue
W/F Pink
W/F Pink
W/F Purple
W/F Purple
W/F Platinum
W/F Platinum



Personal tools