Talk:20.109(S13):Induce protein and evaluate DNA (Day5): Difference between revisions
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Line 23: | Line 23: | ||
|- | |- | ||
|Red | |Red | ||
| | |0.22 | ||
| | |0.51 (D24H) | ||
| | |0.55 | ||
| | |grn, grn, ylw (+hint of grn) | ||
|- | |- | ||
|Orange | |Orange | ||
| | |0.36 | ||
| | |0.36 (D24H) | ||
| | |0.38 | ||
| | |grn, grn, ylw (+hint of grn) | ||
|- | |- | ||
|Yellow | |Yellow | ||
|0.39 | |0.39 | ||
|2.12 (T79P) | |2.12 (T79P) | ||
|3.36 (K77E2--not the chosen mutant) | |3.36 (K77E2--not the chosen mutant)<br>extra is 0.17 | ||
|bright yellow | |bright yellow; ylw (+hint of grn) | ||
|- | |- | ||
|Green | |Green | ||
Line 51: | Line 51: | ||
|0.088 (M71S) | |0.088 (M71S) | ||
|Y, bright ylw | |Y, bright ylw | ||
|- | |- | ||
|Purple | |Purple | ||
| | |0.30 | ||
| | |0.58 (M124S) | ||
| | |0.32 | ||
| | |ylw-grn, ylw-grn, grn | ||
|- | |- | ||
|Platinum | |Platinum | ||
| | |0.52 | ||
| | |0.75 (M124S) | ||
| | |0.38 | ||
| | |ylw-grn, ylw-grn, grn | ||
|- | |- | ||
|} | |} | ||
Line 79: | Line 73: | ||
[[Image:S13_M2D5_TR_gel-Green.jpg|thumb|right|400px|T/R Green]] | [[Image:S13_M2D5_TR_gel-Green.jpg|thumb|right|400px|T/R Green]] | ||
[[Image:S13_M2D5_TR_gel-Blue.jpg|thumb|left|400px|T/R Blue]] | [[Image:S13_M2D5_TR_gel-Blue.jpg|thumb|left|400px|T/R Blue]] | ||
[[Image:S13_M2D5_TR_gel-Purple.jpg|thumb|left|400px|T/R Purple]] | [[Image:S13_M2D5_TR_gel-Purple.jpg|thumb|left|400px|T/R Purple]] | ||
[[Image:S13_M2D5_TR_gel-Platinum.jpg|thumb|center|400px|T/R Platinum]] | [[Image:S13_M2D5_TR_gel-Platinum.jpg|thumb|center|400px|T/R Platinum]] |
Revision as of 07:15, 4 April 2013
Induced pellet example
TR O/N Cultures and Gels
Below you should list the OD600 values of the induced cultures (stock value), as well as your pellet observations. (Is the color dull or bright? More yellow or more green?) The teaching faculty will fill in this information for the cultures needing to be grown overnight.
Group | OD WT | OD D24H/E67K/T79P/M124S | OD Chosen mutant | Pellets yellow? |
---|---|---|---|---|
Example | 0.95 | 1.2 (D24H) | 0.82 (G20P) | Y, bright ylw-grn |
Red | 0.22 | 0.51 (D24H) | 0.55 | grn, grn, ylw (+hint of grn) |
Orange | 0.36 | 0.36 (D24H) | 0.38 | grn, grn, ylw (+hint of grn) |
Yellow | 0.39 | 2.12 (T79P) | 3.36 (K77E2--not the chosen mutant) extra is 0.17 |
bright yellow; ylw (+hint of grn) |
Green | 0.34 | 1.59 (T79P) | 1.16 (M71R) | Bright Yellow |
Blue | 0.035 | 0.036 (M124S) | 0.088 (M71S) | Y, bright ylw |
Purple | 0.30 | 0.58 (M124S) | 0.32 | ylw-grn, ylw-grn, grn |
Platinum | 0.52 | 0.75 (M124S) | 0.38 | ylw-grn, ylw-grn, grn |
Gels
WF O/N Cultures and Gels
Below you should list the OD600 values of the induced cultures (stock value), as well as your pellet observations. (Is the color dull or bright? More yellow or more green?) The teaching faculty will fill in this information for the cultures needing to be grown overnight.
Group | OD WT | OD D24H/E67K/T79P/M124S | OD Chosen mutant | Pellets yellow? |
---|---|---|---|---|
Example | 0.95 | 1.2 (D24H) | 0.82 (G20P) | Y, bright ylw-grn |
Red | ||||
Orange | ||||
Yellow | ||||
Green | ||||
Blue | ||||
Pink | ||||
Purple | ||||
Platinum |
Gels