Talk:20.109(S13):DNA extraction (Day2) - Revision history

AgiStachowiak at 19:10, 10 March 2013

2013-03-10T19:10:35Z

←Older revision		Revision as of 19:10, 10 March 2013
Line 136:		Line 136:
	\|-		\|-
	\|}		\|}
		+
		+	==Bird sample background==
		+
		+	(1) Chain of storage
		+
		+	"Fecal collections are done using a sterile swab into a sterile tube of viral transport
		+	media, which contains a cocktail of antibiotics. The swab is swirled through the specimen,
		+	inserted into the vial, and stored on ice in a cooler while the remaining collections for
		+	that day are being done (usually takes about 2 hours all told back to the lab). Once at the
		+	lab, the samples are immediately transferred to -80C until processing. In the case of
		+	samples coming from Alaska (or any distant field site), the samples are moved directly from
		+	-80C to a dry shipper (maintains the temp at -150C) for air transport, then placed directly
		+	into a -80C when they arrive at our lab. Since all of these have been screened for avian
		+	influenza, the samples were thawed on ice and an aliquote was removed to assess the presence
		+	of AI. The sample is then refrozen at -80C."
		+
		+	(2) Locations
		+
		+	"These are all fecal samples, so they come from land near water. The Alaska samples are
		+	collected off of the fishing docks in the town of Cordova, AK on the Pacific. If you wanted
		+	to be more specific, I believe it's part of Prince William Sound. The MA samples are fecal
		+	pickups from Revere Beach."
		+
		+	(3) Expected species
		+
		+	"The Alaska gulls are primarily glaucous winged gulls, the Revere gulls are primarily herring
		+	gulls and ring billed gulls, with some greater black back gulls and the occasional bonaparte
		+	gull."
		+
		+	(4) Differences between MA and AK pick-ups?
		+
		+	"Other than different people at different locations at different times, no difference at all.
		+	The swabs, VTM, methods, etc are all the same."

Kevin Y. Hu: /* W/F Lab */

2013-02-22T19:53:02Z

W/F Lab

←Older revision		Revision as of 19:53, 22 February 2013
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	\|Eric		\|Eric
	\|732		\|732
-	\|~~Shanti~~	+	\|Shanthi
	\|405		\|405
	\|AK		\|AK
	\|-		\|-
	\|}		\|}

Shannon K. Alford at 17:37, 13 February 2013

2013-02-13T17:37:56Z

←Older revision		Revision as of 17:37, 13 February 2013
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	\|-		\|-
	\|Yellow		\|Yellow
-	\|~~Helen~~	+	\|Jenny W
	\|739		\|739
	\|Karinna		\|Karinna
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	\|Alice		\|Alice
	\|712		\|712
-	\|~~Jenny W~~	+	\|Helen
	\|709		\|709
	\|MA		\|MA

AgiStachowiak: /* W/F Lab */

2013-02-12T17:22:19Z

W/F Lab

←Older revision		Revision as of 17:22, 12 February 2013
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	==W/F Lab==		==W/F Lab==
-

	{\| border="1"		{\| border="1"
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	\|Name		\|Name
	\|Sample #		\|Sample #
-	~~\|AK or MA?~~
	\|Name		\|Name
	\|Sample #		\|Sample #
	\|AK or MA?		\|AK or MA?
	\|-		\|-
-	\|	+	\|Red
-	\|	+	\|Divya
-	\|	+	\|716
-	\|	+	\|Maria
-	\|	+	\|702
-	\|	+	\|MA
-	\|	+
	\|-		\|-
-	\|	+	\|Orange
-	\|	+	\|Katie
-	\|	+	\|712
-	\|	+	\|Amelia
-	\|	+	\|709
-	\|	+	\|MA
-	\|	+
	\|-		\|-
-	\|	+	\|Yellow
-	\|	+	\|Helen
-	\|	+	\|739
-	\|	+	\|Karinna
-	\|	+	\|664
-	\|	+	\|AK
-	\|	+
	\|-		\|-
-	\|	+	\|Green
-	\|	+	\|Kevin
-	\|	+	\|732
-	\|	+	\|Steve
-	\|	+	\|405
-	\|	+	\|AK
-	\|	+
	\|-		\|-
-	\|	+	\|Blue
-	\|	+	\|Emmanuel
-	\|	+	\|716
-	\|	+	\|Sachin
-	\|	+	\|702
-	\|	+	\|MA
-	\|	+
	\|-		\|-
-	\|	+	\|Pink
-	\|	+	\|Alice
-	\|	+	\|712
-	\|	+	\|Jenny W
-	\|	+	\|709
-	\|	+	\|MA
-	\|	+
	\|-		\|-
-	\|	+	\|Purple
-	\|	+	\|Zeinab
-	\|	+	\|739
-	\|	+	\|Jenny V
-	\|	+	\|664
-	\|	+	\|AK
-	\|	+
	\|-		\|-
-	\|	+	\|Grey/Platinum
-	\|	+	\|Eric
-	\|	+	\|732
-	\|	+	\|Shanti
-	\|	+	\|405
-	\|	+	\|AK
-	\|	+
	\|-		\|-
	\|}		\|}

AgiStachowiak at 17:20, 12 February 2013

2013-02-12T17:20:24Z

←Older revision		Revision as of 17:20, 12 February 2013
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	\|Name		\|Name
	\|Sample #		\|Sample #
-	~~\|AK or MA?~~
	\|Name		\|Name
	\|Sample #		\|Sample #
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	\|-		\|-
	\|Red		\|Red
-	\|	+	\|Arturo
-	\|	+	\|716
-	\|	+	\|Nick
-	\|	+	\|702
-	\|	+	\|MA
-	\|	+
	\|-		\|-
	\|Orange		\|Orange
-	\|	+	\|Daphne
-	\|	+	\|712
-	\|	+	\|Stas
-	\|	+	\|709
-	\|	+	\|MA
-	\|	+
	\|-		\|-
	\|Yellow		\|Yellow
-	\|	+	\|Dominique
-	\|	+	\|739
-	\|	+	\|Ebaa
-	\|	+	\|664
-	\|	+	\|AK
-	\|	+
	\|-		\|-
	\|Green		\|Green
-	\|	+	\|Nikki
-	\|	+	\|732
-	\|	+	\|Jesse
-	\|	+	\|405
-	\|	+	\|AK
-	\|	+
	\|-		\|-
	\|Blue		\|Blue
-	\|	+	\|Justin
-	\|	+	\|716
-	\|	+	\|Noa
-	\|	+	\|702
-	\|	+	\|MA
-	\|	+
	\|-		\|-
	\|Pink		\|Pink
-	\|	+	\|Marisol
-	\|	+	\|712
-	\|	+	\|Tiffany
-	\|	+	\|709
-	\|	+	\|MA
-	\|	+
	\|-		\|-
	\|Purple		\|Purple
-	\|	+	\|Sarah
-	\|	+	\|739
-	\|	+	\|Mitali
-	\|	+	\|664
-	\|	+	\|AK
-	\|	+
	\|-		\|-
	\|Grey/Platinum		\|Grey/Platinum
-	\|	+	\|Emily
-	\|	+	\|732
-	\|	+	\|Katie
-	\|	+	\|405
-	\|	+	\|AK
-	\|	+
	\|-		\|-
	\|}		\|}

AgiStachowiak: /* T/R Lab */

2013-02-12T16:02:22Z

T/R Lab

←Older revision		Revision as of 16:02, 12 February 2013
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	\|AK or MA?		\|AK or MA?
	\|-		\|-
-	\|	+	\|Red
	\|		\|
	\|		\|
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	\|		\|
	\|-		\|-
-	\|	+	\|Orange
	\|		\|
	\|		\|
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	\|		\|
	\|-		\|-
-	\|	+	\|Yellow
	\|		\|
	\|		\|
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	\|		\|
	\|-		\|-
-	\|	+	\|Green
	\|		\|
	\|		\|
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	\|		\|
	\|-		\|-
-	\|	+	\|Blue
	\|		\|
	\|		\|
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	\|		\|
	\|-		\|-
-	\|	+	\|Pink
	\|		\|
	\|		\|
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	\|		\|
	\|-		\|-
-	\|	+	\|Purple
	\|		\|
	\|		\|
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	\|		\|
	\|-		\|-
-	\|	+	\|Grey/Platinum
	\|		\|
	\|		\|

AgiStachowiak: New page: Each of you will isolate DNA from a bird stool sample. You will '''''not''''' prepare the same sample as your partner, so please read the table below carefully. ==T/R Lab== {| border="1"...

2013-02-12T15:49:36Z

New page: Each of you will isolate DNA from a bird stool sample. You will '''''not''''' prepare the same sample as your partner, so please read the table below carefully. ==T/R Lab== {| border="1"...

New page

Each of you will isolate DNA from a bird stool sample. You will '''''not''''' prepare the same sample as your partner, so please read the table below carefully.

==T/R Lab==

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==W/F Lab==

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